C4018
Choloylglycine Hydrolase from Clostridium perfringens (C. welchii)
lyophilized powder, ≥100 units/mg protein
Synonym(s):
3α,7α,12α-Trihydroxy-5β-cholan-24-oylglycine amidohydrolase
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| Size/SKU | Availability | Price |
|---|---|---|
1500 units | Please contact Customer Service for Availability | CZK 7,560.00 |
3000 units | Please contact Customer Service for Availability | CZK 14,400.00 |
7500 units | Please contact Customer Service for Availability | CZK 28,800.00 CZK 24,480.00 |
About This Item
CAS Number:
UNSPSC Code:
12352204
NACRES:
NA.54
MDL number:
Specific activity:
≥100 units/mg protein
CZK 7,560.00
Please contact Customer Service for Availability
form
lyophilized powder
Quality Segment
specific activity
≥100 units/mg protein
composition
Protein, ≥50% biuret
shipped in
wet ice
storage temp.
−20°C
Application
Biochem/physiol Actions
The enzyme is an N-terminal nucleophilic (Ntn) hydrolase that catalyzes the hydrolysis of amide bonds, liberates the glycine/taurine moiety from the steroid core and eventually yields unconjugated bile acids. Agents that oxidize thiol groups (e.g., p-mercuribenzoate, iodoacetamide, Hg2+, Cu2+, and Cd2+) have been shown to strongly inhibit bile salt hydrolase (BSH) activity in Clostridium perfringens.[3]
Physical form
Partially purified lyophilized powder containing buffer salts and stabilizer
Other Notes
One unit will hydrolyze 1.0 μmole of glycocholic acid to glycine and cholic acid in 5 min at pH 5.6 at 37 °C.
1 of 1
This Item | |||
|---|---|---|---|
| specific activity ≥100 units/mg protein | specific activity - | specific activity - | specific activity - |
| form lyophilized powder | form powder | form powder | form powder |
| storage temp. −20°C | storage temp. room temp | storage temp. −20°C | storage temp. −20°C |
| shipped in wet ice | shipped in ambient | shipped in dry ice | shipped in dry ice |
| Quality Level 200 | Quality Level 200 | Quality Level - | Quality Level - |
| composition Protein, ≥50% biuret | composition - | composition - | composition - |
signalword
Danger
hcodes
pcodes
Hazard Classifications
Resp. Sens. 1
Storage Class
11 - Combustible Solids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
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