C-27411

Preadipocyte Growth Medium

Basal Medium, 500 ml

• Synonym(s): Recommended with C-39425 or C-39427 (for growth medium) or C-39436 or C-39437 (for Differentiation Medium)
• NACRES: NA.81
• UNSPSC Code: 12352207

Properties

• form: aqueous solution
• packaging: pkg of 500 ml
• technique(s): cell culture | mammalian: suitable
• shipped in: wet ice
• storage temp.: 2-8°C

Description

General description

Preadipocyte Basal Medium (supplements sold separately)

To form a complete Preadipocyte Growth medium, it should be combined with the media supplements: C-39425 or C-39427. To form a complete Preadipocyte Differentiation Medium, it should be combined with the media supplements: C-39436 or C-39437.

Application

Preadipocyte Growth Medium is a low-serum (5% V/V) medium developed for the undifferentiated expansion of human preadipocytes. The media are optimized for primary human cells, but can also be used for murine preadipocytes.Recommended for:Human White Preadipocytes (HWP)

Preparation Note

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Store the Basal Medium at 4 to 8°C in the dark immediately after arrival. Do not freeze the Basal Medium. If stored properly, the medium is stable until the expiry date stated on the label. After adding the supplements to the Basal Medium, the shelf life of the complete medium is 6 weeks at 4 to 8°C. Do not freeze the complete medium. For use, pre-warm only an aliquot of the complete medium and keep the remaining medium refrigerated at 4 to 8°C

Analysis Note

All lots of PromoCell Preadipocyte/Adipocyte Media are subjected to comprehensive quality control tests using primary human white preadipocytes. Each lot is checked for growth promoting activity, adherence rate, and typical morphology of the tested preadipocytes. In addition, the Preadipocyte Differentiation Medium/Adipocyte Nutrition Medium are tested for the capacity to induce the terminal differentiation of preadipocytes into mature adipocytes. Approved in-house lots of media are used as a reference. In addition, all lots of media have been tested for the absence of microbial contaminants (fungi, bacteria, mycoplasma).

Other Notes

Part of a Cell Culture System offered in a variety of formats:Growth Medium (Ready-to-use): Includes Basal Medium and SupplementMixGrowth Medium Kit: Includes Basal Medium and SupplementPackSupplementMix: Contains all media supplements premixed in one vialSupplementPack: Contains all media supplements as individual vials

Safety Information

• Storage Class: 12 - Non Combustible Liquids
• WGK: WGK 1