C-23230

Fibroblast Growth Medium 3

Basal Medium, 500 ml

• Synonym(s): Recommended with C-39345 or C-39350
• NACRES: NA.81
• UNSPSC Code: 12352207

Properties

• form: aqueous solution
• packaging: pkg of 500 ml
• technique(s): cell culture | mammalian: suitable
• shipped in: wet ice
• storage temp.: 2-8°C

Description

General description

Fibroblast Basal Medium 3 (supplements sold separately)

To form a complete medium, it should be combined with the media supplements: C-39345 or C-39350

Application

PromoCell Fibroblast Growth Medium 3 has been developed for the in vitro cultivation of adult Human Cardiac Fibroblasts (HCF). The medium is optimized for primary human cells but can also be used for porcine cardiac fibroblasts.Recommended for:Human Cardiac Fibroblasts (HCF)

Preparation Note

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Final supplement concentrations after addition to the basal medium:Fetal Calf Serum: 0.1 ml / mlBasic Fibroblast Growth Factor (recombinant human): 1 ng / ml Insulin: 5 µg / ml

Store the Basal Medium at 4 to 8°C in the dark immediately after arrival. Do not freeze the Basal Medium. If stored properly, the medium is stable until the expiry date stated on the label. After adding the supplements to the Basal Medium, the shelf life of the complete medium is 6 weeks at 4 to 8°C. Do not freeze the complete medium. For use, pre-warm only an aliquot of the complete medium and keep the remaining medium refrigerated at 4 to 8°C

Analysis Note

All lots of PromoCell Fibroblast Growth Medium 3 are subjected to comprehensive quality control tests using primary Human Cardiac Fibroblasts. Each lot is checked for growth promoting activity, adherence rate, and typical morphology of the tested fibroblasts. Approved inhouse lots of media are used as a reference. In addition, all lots of media have been tested for the absence of microbial contaminants (fungi, bacteria, mycoplasma).

Other Notes

Part of a Cell Culture System offered in a variety of formats:Growth Medium (Ready-to-use): Includes Basal Medium and SupplementMixGrowth Medium Kit: Includes Basal Medium and SupplementPackSupplementMix: Contains all media supplements premixed in one vialSupplementPack: Contains all media supplements as individual vials

Safety Information

• Storage Class: 12 - Non Combustible Liquids
• WGK: WGK 1