For 3-D gel preparation, mix 8 parts of chilled collagen solution with 1 part of 10x PBS or 10x culture medium.
This product is prepared from type I bovine collagen purified and extracted from skin and contains a high monomer content. The raw collagen used to prepare this product has been isolated from a closed herd and purified with a GMP manufacturing process that includes inactivation of any possible prion or viral contamination. As supplied, it is a 6 mg/mL aqueous solution in 0.01 M HCl at a pH of 2.0.
C2124
Bovine Collagen Type I
6 mg/mL, sterile-filtered, (suitable for cell culture and for 3D matrix formation), BioReagent
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50 ML
CZK 26,700.00
About This Item
Biological source:
bovine skin
Form:
liquid
Technique(s):
cell culture | mammalian: suitable using and for 3D matrix formation.
Concentration:
6 mg/mL
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Product Name
Collagen solution from bovine skin, 6 mg/mL, sterile-filtered, (suitable for cell culture and for 3D matrix formation), BioReagent
biological source
bovine skin
Quality Level
sterility
sterile-filtered
product line
BioReagent
form
liquid
packaging
pkg of 50 mL
concentration
6 mg/mL
technique(s)
cell culture | mammalian: suitable using and for 3D matrix formation.
surface coverage
6‑10 μg/cm2
binding specificity
Peptide Source: Collagen
, Peptide Source: Elastin
, Peptide Source: Fibronectin
foreign activity
endotoxin ≤1.0 μmole/min-mg protein
shipped in
wet ice
storage temp.
2-8°C
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1 of 4
This Item | |||
|---|---|---|---|
| sterility sterile-filtered | sterility aseptically processed | sterility - | sterility sterile-filtered |
| biological source bovine skin | biological source bovine (calf) skin | biological source bovine (calf) skin | biological source human cell culture |
| technique(s) cell culture | mammalian: suitable using and for 3D matrix formation. | technique(s) cell culture | mammalian: suitable | technique(s) cell culture | mammalian: suitable | technique(s) cell culture | mammalian: suitable |
| concentration 6 mg/mL | concentration (0.1% solution in 0.1 M acetic acid) | concentration - | concentration 0.3 mg/mL |
| Quality Level 200 | Quality Level 200 | Quality Level 200 | Quality Level 200 |
| form liquid | form solution (0.1% solution in 0.1 M acetic acid) | form solid | form solution |
General description
In mammals, collagen type 1 is a widely expressed fibrous protein. It constitutes to one-third of the total protein in humans. Collagen fibril is the basic component of the tissues containing collagen. It is predominantly expressed in the extracellular matrix, and is also found in arterial walls, tendons, ligaments, skin, cornea, bone and dentin. Collagen structure possesses a triple helix polypeptide strand.
Application
This highly purified solution is suitable for 3-D matrix formation in cell culture. 3-D collagen gels imitate the in vivo cell physiology better than traditional 2D systems and has been proven successful for several cell types including cardian and corneal fibroblasts, depatic stellate cells, and neuroblastoma cells. Such 3-D gels are also useful in studies of mechanotransduction, cell signaling involving the transformation of mechanical signals into biochemical signals
Collagen solution from bovine skin has been used as a substrate in cell adhesion assay.
Biochem/physiol Actions
In 3D environments, cell extensions can use integrins on cell surfaces to activate specific signaling pathways and integran-independent mechanical interactions resulting from the entanglement of matrix fibrils is possible.
Collagen type 1 is a versatile building material required for tissue elasticity and maintains the stability and strength in a variety of tissues. Collagen is also present in byssus thread of invertebrates, which helps in surface attachment. Defective collagen affects the biomechanical property of arterial tissue.
Preparation Note
This product is prepared from type I bovine collagen purified and extracted from skin and contains a high monomer content. The raw collagen used to prepare this product has been isolated from a closed herd and purified with a GMP manufacturing process that includes inactivation of any possible prion or viral contamination. As supplied, it is a 6 mg/mL aqueous solution in 0.01 M HCl at a pH of 2.0.
Other Notes
Collagen is classified into a number of structurally and genetically distinct types. We use the nomenclature proposed by Bornstein and Traub. Be wary of confusing Sigma-type designations with recognized collagen classification types.
Type I collagen differs from other collagens by its low lysine hydroxylation and low carbohydrate composition. As a heterodimer composed of two a1 chains and one a2 chains, it spontaneously forms a triple helix scaffold at neutral pH and 37°C.
Storage Class
10 - Combustible liquids
wgk
nwg
flash_point_f
Not applicable
flash_point_c
Not applicable
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Global Trade Item Number
| SKU | GTIN |
|---|---|
| C2124-50ML | 04061833475157 |
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How can I dissolve this collagen in an aqueous solution? Which buffer should I use?
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How can I dissolve this collagen in an aqueous solution?
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For 3-D gel preparation, mix 8 parts of chilled collagen solution with 1 part of 10x PBS or 10x culture medium.
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