C-12360

Human Pulmonary Fibroblasts (HPF)

500,000 cryopreserved cells

• Synonym(s): Human fibroblast culture
• UNSPSC Code: 41106514
• NACRES: NA.81

Properties

• biological source: human lung
• packaging: pkg of 500,000 cells
• morphology: (fibroblast)
• technique(s): cell culture | mammalian: suitable
• relevant disease(s): pulmonary disorders
• shipped in: dry ice
• storage temp.: −196°C

Description

General description

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Cell Line Origin

Lungs

Application

Primary Human Pulmonary Fibroblasts (HPF) are isolated from human lung tissue and are provided in a cryopreserved format. They are often used for studying lung tissue repair, tissue remodeling after injury, or the response after pulmonary inflammation. Fibroblast disorders like pulmonary fibrosis or hypoxia-induced pulmonary hypertension can also be investigated with cultured HPF.

Quality

Rigid quality control tests are performed for each lot of Human Fibroblasts. They are tested for cell morphology, adherence rate and cell viability. Flow cytometric analyses of the fibroblast specific antigen CD90 are performed for each lot. Growth performance is tested through multiple passages up to 10 or 15 population doublings (PD) under culture conditions without antibiotics and antimycotics. In addition, all cells have been tested for the absence of HIV-1, HIV-2, HBV, HCV, HTLV-1, HTLV-2 and microbial contaminants (fungi, bacteria, and mycoplasma).

Warning

Although tested negative for HIV-1, HIV-2, HBV, HCV, HTLV-1 and HTLV-2, the cells – like all products of human origin – should be handled as potentially infectious. No test procedure can completely guarantee the absence of infectious agents.

Subculture Routine

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Other Notes

Recommended Plating Density: 3500 - 7000 cells per cm²Passage After Thawing: P2Tested Markers: CD90 positiveGuaranteed population doublings: >15

Recommended products

Recommended Primary Cell Culture Media:Link

Disclaimer

RESEARCH USE ONLY. This product is regulated in France when intended to be used for scientific purposes, including for import and export activities (Article L 1211-1 paragraph 2 of the Public Health Code). The purchaser (i.e. enduser) is required to obtain an import authorization from the France Ministry of Research referred in the Article L1245-5-1 II. of Public Health Code. By ordering this product, you are confirming that you have obtained the proper import authorization.

Safety Information

• Storage Class Code: 12 - Non Combustible Liquids
• WGK: WGK 1
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable