C-12271

Human Aortic Endothelial Cells (HAoEC)

500,000 cryopreserved cells

• Synonym(s): HAoEC cells
• NACRES: NA.81
• UNSPSC Code: 41106514
• Biological source: human aorta (thoracic and abdominal)
• Morphology: ( endothelial)
• Relevant disease(s): hypertension; cardiovascular diseases

Properties

• biological source: human aorta (thoracic and abdominal)
• packaging: pkg of 500,000 cells
• morphology: ( endothelial)
• technique(s): cell culture | mammalian: suitable
• relevant disease(s): hypertension; cardiovascular diseases
• shipped in: dry ice
• storage temp.: −196°C

Description

General description

Lot specific orders are not able to be placed through the web. Contact your local sales rep for more details.
Our HAoEC are now also available from HLA-typed and Diabetes Type I and Type II donors.

Application

Primary Human Aortic Endothelial Cells (HAoEC) are isolated from the human ascending (thoracic) and descending (abdominal) aorta and are provided in a cryopreserved format. They are useful for studying vascular diseases such as thrombosis, atherosclerosis, and hypertension as well as for stent-graft compatibility testing.

Biochem/physiol Actions

Aorta

Preparation Note

Subculture Routine –Click here for more information.

Analysis Note

Rigid quality control tests are performed for each lot of Endothelial Cells from large vessels. They are tested for cell morphology, adherence rate, and cell viability. Flow cytometric analyses for cell-type specific markers, e.g. von Willebrand Factor (vWF) and CD31, as well as Dil-Ac-LDL uptake assays are also carried out for each lot. Growth performance is tested through multiple passages up to 15 population doublings (PD) under culture conditions without antibiotics and antimycotics. In addition, all cells have been tested for the absence of HIV-1, HIV-2, HBV, HCV, HTLV-1, HTLV-2 and microbial contaminants (fungi, bacteria, and mycoplasma).

Other Notes

Recommended Plating Density: 5000 - 10000 cells per cm²Passage After Thawing: P2Tested Markers: von Willebrand Factor (vWF) positive, CD31 positive, Dil-Ac-LDL uptake positiveGuaranteed population doublings: >15

Recommended Primary Cell Culture Media:Link

Disclaimer

Although tested negative for HIV-1, HIV-2, HBV, HCV, HTLV-1 and HTLV-2, the cells – like all products of human origin – should be handled as potentially infectious. No test procedure can completely guarantee the absence of infectious agents.

RESEARCH USE ONLY. This product is regulated in France when intended to be used for scientific purposes, including for import and export activities (Article L 1211-1 paragraph 2 of the Public Health Code). The purchaser (i.e. enduser) is required to obtain an import authorization from the France Ministry of Research referred in the Article L1245-5-1 II. of Public Health Code. By ordering this product, you are confirming that you have obtained the proper import authorization.

Safety Information

• Storage Class: 12 - Non Combustible Liquids
• wgk: WGK 1
• flash_point_f: Not applicable
• flash_point_c: Not applicable