B3773
Anti-Human IgG (Fc specific)−Biotin antibody, Mouse monoclonal
clone HP-6017, purified from hybridoma cell culture
Synonym(s):
Monoclonal Anti-Human IgG (Fc specific)
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0.2 ML
CZK 3,510.00
0.5 ML
CZK 6,470.00
About This Item
NACRES:
NA.46
UNSPSC Code:
12352203
Conjugate:
biotin conjugate
Clone:
HP-6017, monoclonal
Application:
ELISA (d)
Citations:
16
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biological source
mouse
Quality Level
conjugate
biotin conjugate
antibody form
purified immunoglobulin
antibody product type
secondary antibodies
clone
HP-6017, monoclonal
form
buffered aqueous solution
species reactivity
rabbit, sheep, horse (IgG), goat, human
technique(s)
direct ELISA: 1:80,000
isotype
IgG2a
shipped in
dry ice
storage temp.
−20°C
target post-translational modification
unmodified
Related Categories
1 of 4
This Item | |||
|---|---|---|---|
| biological source mouse | biological source mouse | biological source mouse | biological source mouse |
| antibody form purified immunoglobulin | antibody form purified immunoglobulin | antibody form purified from hybridoma cell culture | antibody form purified from hybridoma cell culture |
| conjugate biotin conjugate | conjugate biotin conjugate | conjugate FITC conjugate | conjugate FITC conjugate |
| species reactivity rabbit, sheep, horse (IgG), goat, human | species reactivity rabbit | species reactivity - | species reactivity - |
| technique(s) direct ELISA: 1:80,000 | technique(s) direct ELISA: 1:150,000, immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:1,000, western blot (chemiluminescent): 1:200,000-1:400,000 | technique(s) dot immunobinding: 1:32, particle immunofluorescence: 1:16 | technique(s) dot immunobinding: 1:64, particle immunofluorescence: 1:32 |
| isotype IgG2a | isotype IgG1 | isotype IgG1 | isotype IgG2a |
General description
Immunoglobulin IgG is an abundant protein in human serum. The four classes of IgG include IgG1, IgG2, IgG3 and IgG4.. The IgG heavy chain region is mapped to human chromosome 14. Immunoglobulin super-family have a common structure, comprising of two heavy (H) chains and two light (L) chains, held together by disulfide linkages. The heavy chain has one variable N-terminal region and three to four constant (CH1-CH4) C-terminal regions. The L chain comprises of one variable N-terminal region and a constant C-terminal region.
Application
Anti-Human IgG (Fc specific) Biotin antibody, Mouse monoclonal has been used in fluoroimmunoassay of plasma samples.
Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Enzyme-linked immunosorbent assay (1 paper)
Enzyme-linked immunosorbent assay (1 paper)
Monoclonal Anti-Human IgG (Fc specific)-Biotin antibody produced in mouse is suitable for ELISA at a working dilution of 1:80,000. It was used for labeling mouse splenocytes and analysis by flow cytometry.
Biochem/physiol Actions
IgG antibody subtype is the most abundant of serum immunoglobulins of the immune system. It is secreted by B cells and is found in blood and extracellular fluids and provides protection from infections caused by bacteria, fungi and viruses. Maternal IgG is transferred to fetus through the placenta that is vital for immune defense of the neonate against infections. The coupling of biotin to monoclonal Anti-Human IgG (Fc specific) antibody allows for the binding of various labels such as avidin or streptavidin.
IgG1 class is the most abundant and its deficiency results in hypogammaglobulinemia. IgG2 deficiency increases susceptibility to bacterial infections. IgG3 mediates effector functions, and IgG4 is associated with asymptomatic infection. Digestion of IgG by papain results in the generation of fragment antigen binding (Fab). Pepsin digestion of IgG generates fragment crystallisable (Fc). The Fc region of IgG antibody has enormous therapeutic potential and is exploited for the development of therapeutic antibodies.
The antibody is specific for the Fc portion of human IgG and recognizes an epitope common to all human IgG subclasses. This antibody was adopted as an Fc specific reagent in the IUIS/WHO study.
Physical form
Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 1% bovine serum albumin and 15 mM sodium azide
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Storage Class
12 - Non Combustible Liquids
wgk
WGK 2
flash_point_f
Not applicable
flash_point_c
Not applicable
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Intracellular cytokines may model immunoregulation of abacavir hypersensitivity in HIV-infected subjects.
King D, et al.
The Journal of Allergy and Clinical Immunology, 115(5), 1081-1087 (2005)
C Whitney et al.
Infection and immunity, 60(6), 2194-2200 (1992-06-01)
Porphyromonas gingivalis is a suspected pathogen in rapidly progressive periodontitis (RPP). We have determined the anti-P. gingivalis serum immunoglobulin G (IgG) isotype response and avidity and the subclass titer distributions for 30 RPP patients and 30 age-, sex-, and race-matched
Gunnveig Grødeland et al.
PloS one, 8(11), e80008-e80008 (2013-11-19)
Different diseases require different immune responses for efficient protection. Thus, prophylactic vaccines should prime the immune system for the particular type of response needed for protection against a given infectious agent. We have here tested fusion DNA vaccines which encode
María Eugenia Toledo-Romani et al.
Med (New York, N.Y.) (2022-08-24)
SOBERANA 02 has been evaluated in phase I and IIa studies comparing homologous versus heterologous schedule (this one, including SOBERANA Plus). Here, we report results of immunogenicity, safety, and reactogenicity of SOBERANA 02 in a two- or three-dose heterologous scheme
Divya Thiagarajan et al.
Journal of immunology (Baltimore, Md. : 1950), 205(8), 2109-2116 (2020-09-06)
Abs against phosphorylcholine (anti-PC) and Abs against malondialdehyde (anti-MDA) may be protective in chronic inflammation, like atherosclerosis and cardiovascular disease. It is not known how they develop early in life. Ab titers were measured using ELISA in healthy women (n
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Global Trade Item Number
| SKU | GTIN |
|---|---|
| B3773-.2ML | 04061833428399 |
| B3773-.5ML | 04061837514852 |
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