The type of collagenase is Clostridiopeptidase A which is a Collagenase Type I. Please see the link below to review the product datasheet:
https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/116/471/b20222pis-ms.pdf
B20222
Collagenase - Hyaluronidase blend
Tissue dissociation blend, lyophilized powder, suitable for cell culture
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| Size/SKU | Availability | Price |
|---|---|---|
1 vial | Please contact Customer Service for Availability | 3 200,00 CZK |
About This Item
NACRES:
NA.54
UNSPSC Code:
12352204
Specific activity:
(Collagenase activity : 1-5 units/ml in 10 ml HBSS (#H6648; FALGPA assay)), (Hyaluronidase activity : 80-400 units/ml in 10 ml HBSS (#H6648; Hyaluronidase assay))
Concentration:
1 mg/mL (Collagenase)
Quality Segment
form
lyophilized powder
specific activity
(Collagenase activity : 1-5 units/ml in 10 ml HBSS (#H6648; FALGPA assay)), (Hyaluronidase activity : 80-400 units/ml in 10 ml HBSS (#H6648; Hyaluronidase assay))
concentration
1 mg/mL (Collagenase)
technique(s)
cell culture | mammalian: suitable
shipped in
dry ice
storage temp.
-10 to -25°C
General description
The Collagenase-Hyaluronidase blend is a tissue dissociation enzyme cocktail for cell isolation. The tissue dissociation process consists in the release of functional and viable cells from animal tissue, with minimal impact on the cell’s viability. The main reagent used for the tissue dissociation process is Collagenase. Collagenases are enzymes that break down the native collagen that holds animal tissues together. The second enzyme found in our blend is Hyaluronidase. Hyaluronidase is a hyaluronic acid-metabolizing enzyme, who degrades Hyaluronic Acid. Hyaluronic Acid increase tisue and membranes permeability and is one of the main components of the extracellular matrix, contribuing significantly to cell proliferation and migration. Combination of Collagenase and Hyaluronidase is usually used for the isolation of cells from the extracellular matrix.
Biochem/physiol Actions
Collagenase: Effective release of cells from tissue requires the action of collagenase enzymes. Collagenase is activated by four-gram atom calcium (Ca2+) per mole enzyme. The pH optimum is 6.3-8.8. The enzyme is typically used to digest the connective components in tissue samples to liberate individual cells. Collagenase treatment can cause some cells to die. Typically, concentrations varying from 0.1 to 5 mg/mL are used for digestion. The duration of reaction varies from 15 minutes to several hours and yields satisfactory cell dissociation without causing too much cell death. Hyaluronidase: degrades hyaluronan and randomly cleave β-N-acetylhexosamine-(1−4)-glycosidic bonds in hyaluronic acid, chondroitin, and chondroitin sulfates.
Preparation Note
For a working concentration of Collagenase of 1 mg/ml and an enzymatic activity of Hyaluronidase 100 units/ml, reconstitute the lyophilized powder with 10 ml of Hank’s Balanced Salt solution with sodium bicarbonate, without phenol red, calcium chloride and magnesium sulfate (#H6648)
1 of 1
This Item | |||
|---|---|---|---|
| description Tissue dissociation blend, lyophilized powder, suitable for cell culture | description Animal Free Collagenase/Dispase Blend I is a mixture of animal free collagenase, type B, and neutral protease/dispase, and is inteneded to be used when a more potent enzymatic dissociatation solution than collagnaease is desired. | description Animal Free Collagenase, Type B, is designed to have collagenase and secondary proteases similar to Types II collagenase. | description lyophilized powder (from 0.2 μm filtered solution), suitable for cell culture |
| technique(s) cell culture | mammalian: suitable | technique(s) cell culture | mammalian: suitable | technique(s) cell culture | mammalian: suitable | technique(s) cell culture | mammalian: suitable |
| specific activity (Hyaluronidase activity : 80-400 units/ml in 10 ml HBSS (#H6648; Hyaluronidase assay)), (Collagenase activity : 1-5 units/ml in 10 ml HBSS (#H6648; FALGPA assay)) | specific activity - | specific activity - | specific activity ≥800 units/mg solid |
| concentration 1 mg/mL (Collagenase) | concentration - | concentration - | concentration 1 mg/mL |
| form lyophilized powder | form - | form lyophilized | form lyophilized powder (from 0.2 μm filtered solution) |
| shipped in dry ice | shipped in wet ice | shipped in wet ice | shipped in - |
| storage temp. -10 to -25°C | storage temp. 2-8°C | storage temp. 2-8°C | storage temp. −20°C |
signalword
Danger
hcodes
pcodes
Hazard Classifications
Resp. Sens. 1
Storage Class
13 - Non Combustible Solids
wgk
WGK 2
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I am interested in Collagenase-Hyaluronidase Blend (B20222). May I know what type of Collagenase is within it? I will use it for the dissociation process of breast tumor tissue.
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