APPA014
HIV Protease Activity Detection Kit
Synonym(s):
Cut-N-Glow HIV Protease Detection Kit
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| Size/SKU | Availability | Price |
|---|---|---|
1 kit | Please contact Customer Service for Availability | 25 400,00 CZK |
About This Item
NACRES:
NA.32
UNSPSC Code:
12352200
25 400,00 CZK
Please contact Customer Service for Availability
Application
This kit is specific for the HIV Protease, a retroviral aspartyl protease that is critical to viral life-cycle. The Cut-N-Glow approach involves the introduction of a structural distortion into one of the complementary fragments (GFP 11), through the use of a conditionally stable tether, which serves to constrain the N and C termini of GFP 11 closely in space, thereby, diminishing the mutual affinity of the two fragments and blocking protein self-assembly until the tether is cleaved. The distortion can be reversed upon site-specific proteolysis of the tether, resulting in GFP 11 fragment assembly with GFP 1-10, generating reconstituted, functional GFP. Depending on the tether, the chimeric GFP can serve as a substrate for proteases from the three major classes: serine, cysteine, and aspartic acid.
This kit contains sufficient reagents for one 96 well plate (96 tests)
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This Item | |||
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| storage temp. −20°C | storage temp. −20°C | storage temp. −20°C | storage temp. 2-8°C |
| form solution | form - | form - | form - |
| shipped in dry ice | shipped in wet ice | shipped in - | shipped in - |
| Quality Level 200 | Quality Level 300 | Quality Level 200 | Quality Level 200 |
flash_point_f
Not applicable
flash_point_c
Not applicable
Storage Class
12 - Non Combustible Liquids
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