The solubility of this enzyme is tested on the finished product at 1 mL per vial in water. The 100-unit package size will be readily soluble in 1 mL, however, the number of actual units per vial should be reviewed in the lot-specific certificate of analysis. The vial content is typically greater than 100 units. The reconstitution volume would have to be adjusted accordingly in order to achieve a 100 unit per milliliter concentration.
See the link below to review the sample Certificate of analysis:
https://www.sigmaaldrich.com/certificates/sapfs/PROD/sap/certificate_pdfs/COFA/Q14/A6237-100UN0000174833.pdf
A6237
Apyrase from potato
recombinant, expressed in Pichia pastoris, ATPase ≥1000 units/mg protein, lyophilized powder
Synonym(s):
Apyrase from potato, Adenosine 5′-diphosphatase, Adenosine 5′-triphosphatase
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100 UNITS
CZK 3,880.00
500 UNITS
CZK 13,500.00
CZK 3,880.00
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About This Item
Recombinant:
expressed in Pichia pastoris
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recombinant
expressed in Pichia pastoris
Quality Level
form
lyophilized powder
ATPase activity
≥1000 units/mg protein
shipped in
wet ice
storage temp.
−20°C
1 of 4
This Item | |||
|---|---|---|---|
| form lyophilized powder | form lyophilized powder | form lyophilized powder | form lyophilized powder |
| recombinant expressed in Pichia pastoris | recombinant - | recombinant - | recombinant - |
| storage temp. −20°C | storage temp. −20°C | storage temp. −20°C | storage temp. −20°C |
| shipped in wet ice | shipped in wet ice | shipped in - | shipped in - |
| Quality Level 200 | Quality Level 300 | Quality Level 200 | Quality Level 300 |
| ATPase activity ≥1000 units/mg protein | ATPase activity ≥600 units/mg protein | ATPase activity ≥200 units/mg protein | ATPase activity ≥3.0 units/mg protein |
General description
Apyrase, also known as ATP-diphosphohydrolase, is expressed in wide variety of plant and animal tissues. The commercially accessible apyrase enzyme is isolated from potato tubers.
Biochem/physiol Actions
Apyrase hydrolyses the phosphoanhydride bonds of nucleoside tri- and di-phosphates in the presence of divalent cations. It has wide substrate specificity for nucleotides. This property of the enzyme makes it suitable for different biotechnical applications, including DNA sequencing and platelet-aggregation inhibition.
signalword
Danger
hcodes
pcodes
Hazard Classifications
Resp. Sens. 1
Storage Class
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
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Subin Mao et al.
Lab on a chip, 21(16), 3128-3136 (2021-06-29)
Integrins are key players in platelet adhesion and aggregation. Integrin molecular tensions, the forces transmitted by integrin molecules, are regulated by both mechanical and biochemical cues, and the outside-in and inside-out signaling has been extensively studied. While the mechanical properties
Xuan Zhang et al.
European journal of medicinal chemistry, 199, 112397-112397 (2020-05-11)
Targeting BCL-XL via PROTACs is a promising strategy in reducing BCL-XL inhibition associated platelet toxicity. Recently, we reported potent BCL-XL PROTAC degraders that recruit VHL or CRBN E3 ligase. However, low protein expression or mutation of the responsible E3 ligase
Celia Cordero-Sanchez et al.
Blood advances, 6(15), 4471-4484 (2022-06-14)
Store-operated Ca2+-entry is a cellular mechanism that governs the replenishment of intracellular stores of Ca2+ upon depletion caused by the opening of intracellular Ca2+-channels. Gain-of-function mutations of the 2 key proteins of store-operated Ca2+-entry, STIM1 and ORAI1, are associated with
Methylotrophic yeast Pichia pastoris as a host for production of ATP-diphosphohydrolase (apyrase) from potato tubers (Solanum tuberosum).
Nourizad N, et al.
Protein Expression and Purification, 27(2), 229-237 (2003)
Cloning, sequencing, and expression of a human brain ecto-apyrase related to both the ecto-ATPases and CD39 ecto-apyrases1.
Smith T M & Kirley T L
Biochimica et Biophysica Acta, 1386(1), 65-78 (1998)
Related Content
Global Trade Item Number
| SKU | GTIN |
|---|---|
| A6237-500UN | 04061832754611 |
| A6237-100UN | 04061832754604 |
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Hello. Is the concentration of 100 units in 1 mL of water? If not, how can I make the dilution to obtain 100 units? Thanks for you attention! Guilherme
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