The product is not tested for microbial contamination. Instead, a fluorometric toxicity assay is conducted. This involves growing three mammalian cell lines in the presence of the test product. After 7 days of incubation, the fluorescence is measured, and it is directly proportional to cell density.
A4034
(+)-Sodium L-ascorbate
powder, BioReagent, suitable for cell culture
Synonym(s):
L(+)-Ascorbic acid sodium salt, Vitamin C sodium salt
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100 G
CZK 2,360.00
500 G
CZK 5,730.00
1 KG
CZK 8,750.00
About This Item
Empirical Formula (Hill Notation):
C6H7NaO6
CAS Number:
Molecular Weight:
198.11
NACRES:
NA.75
PubChem Substance ID:
eCl@ss:
34058010
UNSPSC Code:
12352205
EC Number:
205-126-1
MDL number:
Beilstein/REAXYS Number:
3767246
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InChI key
PPASLZSBLFJQEF-RXSVEWSESA-M
InChI
1S/C6H8O6.Na/c7-1-2(8)5-3(9)4(10)6(11)12-5;/h2,5,7-10H,1H2;/q;+1/p-1/t2-,5+;/m0./s1
SMILES string
[Na+].OC[C@H](O)[C@H]1OC(=O)C(O)=C1[O-]
biological source
synthetic (organic)
product line
BioReagent
assay
≥98%
form
powder
mol wt
Mw 198.11 g/mol
technique(s)
cell culture | mammalian: suitable, cell culture | plant: suitable
color
white to off-white
mp
220 °C (dec.) (lit.)
solubility
H2O: 50 mg/mL
Quality Level
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1 of 4
This Item | |||
|---|---|---|---|
| technique(s) cell culture | mammalian: suitable, cell culture | plant: suitable | technique(s) HPLC: suitable, gas chromatography (GC): suitable | technique(s) gas chromatography (GC): suitable, liquid chromatography (LC): suitable | technique(s) HPLC: suitable |
| assay ≥98% | assay - | assay - | assay ≥98% |
| biological source synthetic (organic) | biological source - | biological source synthetic | biological source synthetic |
| form powder | form - | form solid | form crystalline |
| solubility H2O: 50 mg/mL | solubility - | solubility - | solubility - |
| mp 220 °C (dec.) (lit.) | mp 220 °C (dec.) (lit.) | mp 220 °C (dec.) (lit.) | mp 220 °C (dec.) (lit.) |
General description
(+)-Sodium L-ascorbate (SA) is a potential antioxidant and is a derivative of vitamin C (L-ascorbic acid).[1] It is widely used as a food additive. In addition, SA enhances growth retardation activity of bleomycin sulfate, R020-1724 (an inhibitor of cyclic nucleotide phosphodiesterase), prostaglandin E1 and sodium butyrate agents on neuroblastoma cells in culture. Elevated levels of SA induces two-stage urinary bladder carcinogenesis in rats. sodium L-ascorbate in combination with collagenogenesis inhibitors is used to treat dermal scars and keloids.[1]
Application
(+)-Sodium L-ascorbate has been used:
- to produce H2O2 in cell culture media
- as a reducing agent for a cell-permeable artificial electron donor, N,N,N′,N′-tetramethyl-p-phenylenediamine (TMPD)
- to study its effect on production of collagen and elastic fibers in fibroblasts cultures derived from normal human skin and dermal fat[1]
Used for preparation of plant extracts; reductant for the isolation of chloroplasts.
Biochem/physiol Actions
Antioxidant
Storage Class
11 - Combustible Solids
wgk
WGK 1
ppe
Eyeshields, Gloves, type N95 (US)
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Sodium ascorbate potentiates the growth inhibitory effect of certain agents on neuroblastoma cells in culture
Prasad KN, et al.
Proceedings of the National Academy of Sciences of the USA, 76(2), 829-832 (1979)
Artefacts in cell culture: pyruvate as a scavenger of hydrogen peroxide generated by ascorbate or epigallocatechin gallate in cell culture media
Long LH and Halliwell B
Biochemical and Biophysical Research Communications, 388(4), 700-704 (2009)
Artefacts in cell culture: alpha-Ketoglutarate can scavenge hydrogen peroxide generated by ascorbate and epigallocatechin gallate in cell culture media
Long LH and Halliwell B
Biochemical and Biophysical Research Communications, 406(1), 20-24 (2011)
Promoting effects of sodium L-ascorbate on two-stage urinary bladder carcinogenesis in rats
Fukushima S, et al.
Cancer Research, 43(9), 4454-4457 (1983)
The putative Drp1 inhibitor mdivi-1 is a reversible mitochondrial complex I inhibitor that modulates reactive oxygen species
Bordt EA, et al.
Developmental Cell, 40(6), 583-594 (2017)
Articles
Importance and uses of ascorbate in serum-free eukaryotic, including hybridoma and Chinese Hamster Ovary (CHO) cell, cultures
Antioxidants protect biological systems from oxidative damage produced by oxygen-containing free radicals and from redoxactive transition metal ions such as iron, copper, and cadmium.
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