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Merck

A3574

Acrylamide/Bis-acrylamide solution

30%, BioReagent, suitable for electrophoresis, 29:1

Synonym(s):

30% Acrylamide/Bisacrylamide 29:1, Polyacrylamide solution

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100 ML

CZK 2,610.00

5 X 100 ML

CZK 6,820.00

5 L

CZK 17,765.00

CZK 2,610.00

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About This Item

UNSPSC Code:
41105319
PubChem Substance ID:
329770837
NACRES:
NA.25
MDL number:
MFCD00080848

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Product Name

Acrylamide/Bis-acrylamide, 30% solution, BioReagent, suitable for electrophoresis, 29:1

sterility

0.2 μm filtered

Quality Level

200

product line

BioReagent

form

liquid

feed ratio

29:1

technique(s)

electrophoresis: suitable

suitability

suitable for electrophoresis

foreign activity

Protease, none detected

storage temp.

2-8°C

SMILES string

NC(=O)C=C.C=CC(=O)NCNC(=O)C=C

InChI

1S/C7H10N2O2.C3H5NO/c1-3-6(10)8-5-9-7(11)4-2;1-2-3(4)5/h3-4H,1-2,5H2,(H,8,10)(H,9,11);2H,1H2,(H2,4,5)

InChI key

OCQZXMCGTAWGEQ-UHFFFAOYSA-N

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1 of 4

This Item
A3449A9926A2917
Acrylamide/Bis-acrylamide, 30% solution BioReagent, suitable for electrophoresis, 29:1

Sigma-Aldrich

A3574

Acrylamide/Bis-acrylamide, 30% solution

Acrylamide/Bis-acrylamide, 30% solution BioReagent, suitable for electrophoresis, 19:1

Sigma-Aldrich

A3449

Acrylamide/Bis-acrylamide, 30% solution

Acrylamide/Bis-acrylamide, 40% solution BioReagent, suitable for electrophoresis, 19:1

Sigma-Aldrich

A9926

Acrylamide/Bis-acrylamide, 40% solution

Acrylamide/Bis-acrylamide BioReagent, 19:1 (ratio), Molecular Biology

Sigma-Aldrich

A2917

Acrylamide/Bis-acrylamide

form

liquid

form

liquid

form

liquid

form

powder

technique(s)

electrophoresis: suitable

technique(s)

electrophoresis: suitable

technique(s)

electrophoresis: suitable

technique(s)

-

suitability

suitable for electrophoresis

suitability

suitable for electrophoresis

suitability

suitable for electrophoresis

suitability

-

feed ratio

29:1

feed ratio

19:1

feed ratio

19:1

feed ratio

19:1 (ratio)

foreign activity

Protease, none detected

foreign activity

Protease, none detected

foreign activity

-

foreign activity

DNAse (exonuclease), NICKase (endonuclease), RNAse, and protease, none detected

sterility

0.2 μm filtered

sterility

0.2 μm filtered

sterility

0.2 μm filtered

sterility

-

General description

Polyacrylamide gels are prepared using acrylamide and bis-acrylamide, where bis-acrylamide is a cross-linking agent. On the other hand, acrylamide is a water soluble monomer which helps in forming a transparent stable insoluble gel.

Application

These 30% (w/w) acrylamide/bis-acrylamide solutions are available at the most common ratios for use in protein and nucleic acid electrophoresis. The concentration is based on the total weight of both the acrylamide and bis-acrylamide.
Acrylamide/Bis-acrylamide, 30% solution has been used as a constituent in the preparation of 4% stacking gel and 10% resolving gel during sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) gel electrophoresis. It has also been used as a precursor for polyacrylamide lid fabrication by photopatterning methods.

Preparation Note

Solution prepared from electrophoresis grade acrylamide and bis-acrylamide in ultra-pure water.

pictograms

Health hazardExclamation mark
GHS08,GHS07

signalword

Danger

Hazard Classifications

Acute Tox. 4 Inhalation - Acute Tox. 4 Oral - Carc. 1B - Eye Irrit. 2 - Muta. 1B - Repr. 2 - Skin Irrit. 2 - Skin Sens. 1 - STOT RE 1 Oral

target_organs

Peripheral nervous system

Storage Class

6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects

wgk

WGK 3

ppe

Eyeshields, Faceshields, Gloves, type ABEK (EN14387) respirator filter

Regulatory Listings

Regulatory Listings are mainly provided for chemical products. Only limited information can be provided here for non-chemical products. No entry means none of the components are listed. It is the user’s obligation to ensure the safe and legal use of the product.

79-06-1

CAS No.

79-06-1

CAS No.

Choose from one of the most recent versions:

Certificates of Analysis (COA)

Lot/Batch Number
0000430113
0000430113
0000409071
0000409071
0000325128

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Subcellular western blotting of single cells
Yamauchi KA and Herr AE
Microsystems & Nanoengineering, 3, 16079-16079 (2017)
Separation and Detection of Phosphorylated and Nonphosphorylated BvgA, a Bordetella pertussis Response Regulator, in vivo and in vitro
Chen Q, et al.
Bio-protocol, 3(22) (2013)
Ana Techniqs in Biotechnology (2011)
Thi Cuc Mai et al.
Scientific reports, 10(1), 5724-5724 (2020-04-03)
Communication technologies based on radiofrequency (RF) propagation bring great benefits to our daily life. However, their rapid expansion raises concerns about possible impacts on public health. At intensity levels below the threshold to produce thermal effects, RF exposure has also
Preparation and properties of acrylamide gel for use in electrophoresis.
Raymond S and Wang YJ
Analytical Biochemistry, 1(4-5), 391-396 (1960)
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Related Content

Global Trade Item Number

SKUGTIN
A3574-5L04061838254610
A3574-100ML04061838254603
A3574-5X100ML04061838254627

Questions

1–2 of 2 Questions  

  1. How can I determine the shelf life / expiration / retest date of this product?

    1 answer

    1. If this product has an expiration or retest date, it will be shown on the Certificate of Analysis (COA, CofA). If there is no retest or expiration date listed on the product's COA, we do not have suitable stability data to determine a shelf life. For these products, the only date on the COA will be the release date; a retest, expiration, or use-by-date will not be displayed.
      For all products, we recommend handling per defined conditions as printed in our product literature and website product descriptions. We recommend that products should be routinely inspected by customers to ensure they perform as expected.
      For products without retest or expiration dates, our standard warranty of 1 year from the date of shipment is applicable.
      For more information, please refer to the Product Dating Information document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/418/501/product-dating-information-06-25-mk.pdf

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  2. How is shipping temperature determined? And how is it related to the product storage temperature?

    1 answer

    1. Products may be shipped at a different temperature than the recommended long-term storage temperature. If the product quality is sensitive to short-term exposure to conditions other than the recommended long-term storage, it will be shipped on wet or dry-ice. If the product quality is NOT affected by short-term exposure to conditions other than the recommended long-term storage, it will be shipped at ambient temperature. As shipping routes are configured for minimum transit times, shipping at ambient temperature helps control shipping costs for our customers. For more information, please refer to the Storage and Transport Conditions document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/316/622/storage-transport-conditions-mk.pdf

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