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A3422

Achromopeptidase from bacteria

partially purified powder, ≥20,000 units/mg solid

Synonym(s):

ACP, Achromopeptidase

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25000 UNITS

CZK 15,000.00

50000 UNITS

CZK 27,800.00

CZK 15,000.00

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About This Item

UNSPSC Code:
12352204
NACRES:
NA.54
EC Number:
3.4.21.50 (BRENDA, IUBMB)
MDL number:
MFCD00130333
Specific activity:
≥20,000 units/mg solid

Key Documents

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form

partially purified powder

Quality Level

200

specific activity

≥20,000 units/mg solid

mol wt

27 kDa

foreign activity

Collagenase, present

storage temp.

−20°C

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A3547P9752G0751
Achromopeptidase from bacteria partially purified powder, ≥20,000 units/mg solid

Sigma-Aldrich

A3422

Achromopeptidase from bacteria

Achromopeptidase from bacteria lyophilized powder, ≥1,000 units/mg solid

Sigma-Aldrich

A3547

Achromopeptidase from bacteria

Phosphoriboisomerase from spinach Type I, partially purified powder, ≥40 units/mg protein (biuret)

Sigma-Aldrich

P9752

Phosphoriboisomerase from spinach

β-Glucuronidase from Helix pomatia Type H-1, partially purified powder, ≥300,000 units/g solid

Sigma-Aldrich

G0751

β-Glucuronidase from Helix pomatia

specific activity

≥20,000 units/mg solid

specific activity

≥1,000 units/mg solid

specific activity

≥40 units/mg protein (biuret)

specific activity

≥300,000 units/g solid

form

partially purified powder

form

lyophilized powder

form

partially purified powder

form

partially purified powder

mol wt

27 kDa

mol wt

27 kDa

mol wt

-

mol wt

-

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−20°C

Quality Level

200

Quality Level

200

Quality Level

200

Quality Level

200

foreign activity

Collagenase, present

foreign activity

-

foreign activity

-

foreign activity

-

Application

Achromopeptidase is useful for lysis of Gram-positive bacteria that are resistant to lysozyme. Achromopeptidase, from Sigma, has been used for the isolation and molecular characterization of skin and soft-tissue bacterial infections[1]. Product A3422 is from bacteria and is a partially purified powder containing lactose.
Achromopeptidase from bacteria may be used:
  • in the homogenization step for genomic DNA extraction from microbial biofilm
  • in the lysis of gram-positive strains(
  • for DNA extraction from Nocardia strains

Biochem/physiol Actions

Achromopeptidase is a lysyl endopeptidase that has a molecular weight of approximately 27 kDa. It works best between pH 8.5 -9. Approximately 500-1,500 μn/ml achromopetidase can be used to lyse cells at a density of OD600 = 0.6 over 2 hours at 37 °C.
Achromopeptidase is a bacteriolytic enzyme and belongs to serine protease family. It is effective for the lysis of gram positive bacteria compared to lysozyme. Achromopeptidase activity is inhibited in the presence of heavy metals. Achromopeptidase based inhibition assay may be useful for screening the presence of heavy metals.

Physical form

Partially purified powder.

Other Notes

One unit will lyse 0.4 μg of Micrococcus lysodeikticus per minute by turbidimetric detection at 600 nm when suspended in buffer at pH 8.0 at 37 °C.

pictograms

Health hazard
GHS08

signalword

Danger

hcodes

H334

Hazard Classifications

Resp. Sens. 1

Storage Class

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

dust mask type N95 (US), Eyeshields, Faceshields, Gloves

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Certificates of Analysis (COA)

Lot/Batch Number
SLCP7173
SLCP7173
SLCN5444
SLCK0436
SLBT0140

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Find documentation for the products that you have recently purchased in the Document Library.

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Microbial ecosystem analysis in root canal infections refractory to endodontic treatment
Henriques LC, et al.
Journal of Endodontics, 42(8), 1239-1245 (2016)
Metagenomic analysis of an ecological wastewater treatment plant?s microbial communities and their potential to metabolize pharmaceuticals
Balcom IN, et al.
F1000Research, 5, 844-846 (2016)
Achromopeptidase for lysis of anaerobic gram-positive cocci.
Ezaki T and Suzuki S
Journal of Clinical Microbiology, 16(5), 844-846 (1982)
High Intraspecific Genetic Diversity of Nocardia brasiliensis, a Pathogen Responsible for Cutaneous Nocardiosis Found in France: Phylogenetic Relationships by Using sod and hsp65 Genes
Kosova-Maali D, et al.
BioMed Research International, 2018 (2018)
Near real-time inhibitive assay for heavy metals using achromopeptidase
Shukor MY, et al.
Indian Journal of Biotechnology, 13, 398-403 (2014)
View All Related Papers

Protocols

Enzymatic Assay of Achromopeptidase

To measure achromopeptidase activity, this procedure uses bacterial cells and a turbidimetric rate assay. Turbidity is measured at 600 nm using a spectrophotometer.

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Achromopeptidase from Achromobacter lyticus (A3422)

QC Methods

Global Trade Item Number

SKUGTIN
A3422-50KU04061832699523
A3422-25KU04061832699509

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