A2580

Angiotensin Converting Enzyme from porcine kidney

lyophilized powder, ≥10 units/mg protein (Bradford)

• Synonym(s): ACE, Peptidyl-dipeptidase A
• CAS Number: 9015-82-1
• UNSPSC Code: 12352200
• NACRES: NA.32
• EC Number: 3.4.15.1 (BRENDA, IUBMB)
• MDL number: MFCD00130325
• eCl@ss: 42010109
• Specific activity: ≥10 units/mg protein (Bradford)

Properties

• form: lyophilized powder
• Quality Segment: 200
• specific activity: ≥10 units/mg protein (Bradford)
• UniProt accession no.: A0A480IQM9
• shipped in: dry ice
• storage temp.: −20°C
• Gene Information: pig ... ACE(613133)

Description

General description

Angiotensin converting enzyme (ACE) is encoded by the gene, mRNA-decapping enzyme subunit 1 (DCP1). It is a zinc metalloprotease, which belongs to the M2 family.

ACE is a monomer with molecular weight of ~170 kDa
pH range for activity: 7-8.5
Temperature optimum: 37 °C
Zinc is required for activity
Inhibitors: captopril, enalaprilat, lisinopril etc. (1-10 μM)

Application

Angiotensin converting enzyme from porcine kidney has been used in in vitro angiotensin-converting enzyme (ACE) inhibition assay.

Biochem/physiol Actions

Angiotensin converting enzyme (ACE) catalyzes the conversion of angiotensin I to angiotensin II, which regulates the fluid-electrolyte balance and systemic blood pressure. ACE inhibits the vasodilator, bradykinin. ACE inhibitors are used to treat high blood pressure.

Removes C-terminal dipeptides from susceptible substrates, e.g., angiotensin I and bradykinin.

Physical form

Lyophilized powder containing Tris buffer salts.

Other Notes

One unit will produce 1.0 ·μmole of hippuric acid from Hippuryl-His-Leu per min in 50 mM HEPES and 300 mM NaCl at pH 8.3 at 37 °C.

Safety Information

• pictograms: GHS08
• signalword: Danger
• hcodes: H334
• pcodes: P261 - P284 - P501
• Hazard Classifications: Resp. Sens. 1
• Storage Class: 11 - Combustible Solids
• wgk: WGK 3
• flash_point_f: Not applicable
• flash_point_c: Not applicable
• ppe: Eyeshields, Gloves, type N95 (US)