Accuspin tubes are obtained from an outside supplier. Their exact dimensions are not available but should approximate the dimensions of a standard 50 ml centrifuge tube. Standard dimensions are generally 29.1 mm in diameter X 114.4 mm long.
A2055
ACCUSPIN™ Tubes Sterile, 50 mL Capacity
radiation sterilized tube fitted with a high density polyethylene barrier
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| Size/SKU | Availability | Price |
|---|---|---|
10 ea | Please contact Customer Service for Availability | CZK 4,510.00 |
About This Item
NACRES:
NA.75
UNSPSC Code:
12352207
Quality Segment
sterility
sterile; irradiated
form
tubes
application(s)
hematology
histology
storage temp.
15-25°C
General description
ACCUSPIN™ Tubes are polypropylene radiation sterilized tube fitted with a high density polyethylene barrier. Each tube will accept 15 mL of the density gradient.
Application
ACCUSPIN™ Tubes Sterile, 50 mL Capacity is intended for use with the density gradient separation medium Histopaque™ -1077:
- in the isolation of lymphocytes and other mononuclear cells.
- isolation of cord blood–derived endothelial progenitor cells.[1]
- isolate mononuclear cell layer from bone marrow samples.
- suitable for the purification of human bone marrow aspirates for the investigation of the therapeutic value of BET inhibitors for acute myeloid leukemia.
Biochem/physiol Actions
Following the addition of Histopaque-1077 to theACCUSPIN Tube, a brief centrifugation places theHistopaque-1077 below the frit. The blood sample canbe added to the top chamber of the tube without risk ofmixing with the Histopaque-1077 in the lower chamberunder the frit. On subsequent centrifugation the wholeblood descends through the frit to contact with theHistopaque-1077. The elements of greater densitydisplace a volume of Histopaque-1077 above the fritgiving a clear separation of the blood components. Theerythrocytes aggregate and the granulocytes becomeslightly hypertonic, increasing their sedimentation rate,resulting in pelleting at the bottom of the ACCUSPINTube. Lymphocytes and other mononuclear cells, e.g.,monocytes, remain at the plasma/Histopaque-1077interface. This dense band of mononuclear cells maybe collected by pouring off the contents of the upperchamber or by means of a pipette. Erythrocytecontamination is avoided due to the barrier between thechambers.
Preparation Note
Package contains 10 tubes
Legal Information
Accuspin is a trademark of Sigma-Aldrich Co. LLC
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This Item | |||
|---|---|---|---|
| sterility sterile; irradiated | sterility sterile; irradiated | sterility aseptically filled | sterility aseptically filled |
| form tubes | form tubes | form solution | form solution |
| Quality Level 200 | Quality Level 200 | Quality Level 400 | Quality Level 400 |
| storage temp. 15-25°C | storage temp. 15-25°C | storage temp. 2-8°C | storage temp. 2-8°C |
| application(s) hematology | application(s) hematology | application(s) hematology | application(s) hematology |
signalword
Danger
hcodes
Hazard Classifications
Resp. Sens. 1 - Skin Sens. 1
Storage Class
10 - Combustible liquids
flash_point_f
Not applicable
flash_point_c
Not applicable
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1 answer-
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How should a 4-20% sucrose gradient be used to reduce platelet contamination, as recommended in the user manual?
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Sucrose exerts an osmotic pressure of 300 mOsm at a concentration of about 0.25M, which is iso-osmotic with the contents of most mammalian cells. This concentration is approximately 81.25 grams/L or 8.125%, as per protocols used to remove platelets. Recommended procedures for removing platelets:
Perform Procedure 1077 or 1077/1119.
Collect the cell band and layer it over a second gradient.
A. Bottom layer = 1077-1
B. Top layer = 8.125% sucrose solution (prepared in deionized water).
C. Layer the cell suspension on top of the gradient formed in steps 1-2.
D. Use a 300 g spin for 10-15 minutes.
E. Depending upon the original volume of the cell suspension, times and speed may have to be adjusted to maximize cell recovery.
F. Use separate tubes for mononuclear and polymorphonuclear cells.Helpful?
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Is Accuspin (A2055) suitable for use with canine blood?
1 answer-
The suggestion is to make a decision based on individual circumstances.If currently using a plain centrifuge tube for collecting PBMCs from canine blood using a single density gradient, the Accuspin Tubes can be used for the same collection, provided that the G force does not exceed 1000G and a single density gradient is used.
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Does the Accuspin Tubes Product #A2055, which are prefilled with a frit, also contain EDTA or another anticoagulant?
1 answer-
None of the Accuspin spin tubes (filled or unfilled) contain any anticoagulant. It is expected that when blood is drawn, it is typically drawn into a vacuum tube that already contains an anticoagulant or it is necessary to add anticoagulant immediately after the blood draw is completed. The most common anticoagulants used are EDTA and Heparin.
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Are Accuspin tubes compatible with blood that has been anticoagulated with ACD-A?
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Certainly, Accuspin tubes are suitable for use with any blood containing an anticoagulant, with fresher blood being preferable. Regardless of the anticoagulant used, the anticoagulant is essentially washed away during the steps to remove the density gradient from the collected white blood cells. With ACD-A, the cell band is wider at the interface and the cells are more dispersed over a larger area, possibly due to the dextrose/glucose in the formulation. When using ACD-A, a somewhat unusual cell band is produced after the tubes are removed from the centrifuge. In comparison, cell bands with heparin, EDTA, sodium citrate, and other typical anticoagulants typically provide a rather narrow cell band when viewed from the side of the Accuspin tube.
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Can I remove the Frit after collecting the mononuclear cells to recover the granulocytes?
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When using the Accuspin System to collect Peripheral Blood Mononuclear Cells (PBMCs), there is no expectation that the neutrophils can be recovered. Although it is possible to dislodge the frit, some granulocytes will be suspended in the lower layers of the density gradient. The remaining granulocytes will appear as a gray layer on top of the packed red blood cells.
In theory, the end-user may be able to collect this buffy coat that sits atop the packed red blood cell layer. It is recommended to either perform a lysis step to lyse the red blood cells, or run the cells back over another density gradient - such as Histopaque 11191 that has a density of 1.119 g/ml.
Instead of performing this 2-step procedure that has not been validated, the more logical option would be to simply run the whole peripheral blood over a 2-step discontinuous gradient. Currently, Histopaque 10771 and Histopaque 11191, are offered. The Histopaque 11191 instructions for use are available in the DOCUMENTATION section in the 'More Documents' tab of the product detail page:
https://www.sigmaaldrich.com/product/sigma/a2055#product-documentation
https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/193/872/a2055pis.pdfHelpful?
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