This product is filled by density, with a target fill volume of 0.5 ml for the 25mg and 2.0 ml for the 100mg package sizes.
A1602
Amyloglucosidase from Aspergillus niger
ammonium sulfate suspension, ≥40 units/mg protein
Synonym(s):
1,4-α-D-Glucan glucohydrolase, Exo-1,4-α-glucosidase, Glucoamylase
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A1602-25MG
CZK 3,890.00
A1602-100MG
CZK 11,800.00
CZK 3,890.00
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About This Item
CAS Number:
UNSPSC Code:
12352204
NACRES:
NA.54
MDL number:
Specific activity:
≥40 units/mg protein
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form
ammonium sulfate suspension
Quality Level
specific activity
≥40 units/mg protein
shipped in
wet ice
storage temp.
2-8°C
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Related Categories
1 of 4
This Item | |||
|---|---|---|---|
| specific activity ≥40 units/mg protein | specific activity 30-60 units/mg protein (biuret) | specific activity - | specific activity ≥10 unit/mg solid, ≥20 units/mg protein |
| form ammonium sulfate suspension | form lyophilized powder | form liquid | form lyophilized |
| shipped in wet ice | shipped in - | shipped in - | shipped in wet ice |
| storage temp. 2-8°C | storage temp. −20°C | storage temp. 2-8°C | storage temp. −20°C |
| Quality Level 200 | Quality Level 200 | Quality Level 100 | Quality Level 100 |
General description
Amyloglucosidase, a disaccharide enzyme is a member of the alpha-glucosidase type. It is produced by several species of Aspergillus genus.
Application
Amyloglucosidase from Aspergillus niger is used to hydrolyze α-D-glucosides. It may be used in the brewing of beer and in the production of bread and juices. Amyloglucosidase, from Sigma, has been used to hydrolyze glycogen into glucose monomers in order to study lipid accumulation in skeletal muscle [1].
Amyloglucosidase from Aspergillus niger has been used to liberate free glucose in the Belgica antarctica larva homogenate. It has also been used in digestive enzyme mixture for starch digestion.
Biochem/physiol Actions
Amyloglucosidase from Aspergillus niger is capable of hydrolyzing the α-D-(1-4), the α-D-(1-6), and the α-D-(1-3) glucosidic bonds of oligosaccharides [2].
Physical form
Suspension in 3.2 M (NH4)2SO4 solution, pH approx. 6.0
Other Notes
One unit will liberate 1.0 mg of glucose from starch in 3 min at pH 4.5 at 55 °C.
View more information on enzymes for complex carbohydrate analysis at www.sigma-aldrich.com/enzymeexplorer
Storage Class
12 - Non Combustible Liquids
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)
Regulatory Listings
Regulatory Listings are mainly provided for chemical products. Only limited information can be provided here for non-chemical products. No entry means none of the components are listed. It is the user’s obligation to ensure the safe and legal use of the product.
7783-20-2
CAS No.
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Insulin is a vital part of diabetes treatment, whereas glucagon is primarily used to treat insulin-induced hypoglycemia. However, glucagon is suggested to have a central role in the regulation of body weight, which would be beneficial for diabetic patients. Since
Survival and energetic costs of repeated cold exposure in the Antarctic midge, Belgica antarctica: a comparison between frozen and supercooled larvae
Teets NM, et al.
The Journal of Experimental Biology, 214(5), 806-814 (2011)
Articles
Instructions for working with enzymes supplied as ammonium sulfate suspensions
Protocols
This procedure may be used for the determination of Amyloglucosidase activity using starch as the substrate.
Related Content
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What volume of ammonium sulfate is the 25mg suspended in?
1 answer-
Helpful?
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How can a 1mg/mL solution of amyloglucosidase A1602 be prepared in acetate buffer?
1 answer-
The product A1602 is in an ammonium sulfate solution. Here's how you can work with this product:
- If the ammonium sulfate might interfere with the reaction(s):
1. Remove the enzyme from the refrigerator, ensuring that the ammonium sulfate suspensions are not frozen.
2. Gently invert the vial or bottle to produce a milky suspension. Avoid using harsh treatments like vortexing or sonicating, as they may denature some of the enzyme.
3. Use a pipette and sterile wide-bore pipette tip to remove a portion of the suspension.
4. Transfer the portion to a clean microcentrifuge or centrifuge tube.
5. Pellet the enzyme by centrifuging at approximately 10,000 – 15,000 x g for 10 minutes, if possible, using a refrigerated microcentrifuge set to a temperature between 2 °C and 8 °C.
6. Carefully remove as much clear supernatant as possible, retaining it; it is not necessary to remove absolutely all of the ammonium sulfate solution.
7. Dissolve the pellet by adding an appropriate amount of reaction buffer.
8. Assay the supernatant and the solution from the pellet for protein content and/or enzyme activity.- If the ammonium sulfate will not interfere with the reaction(s), follow the same steps as above.
It's important to note that for an ammonium sulfate suspension, most of the enzyme will be in solid form, and only negligible amounts of enzyme will likely be in the ammonium sulfate solution.
Alternatively, there are several Amyloglucosidase enzymes available in solid form (e.g., Product A7420 and A9228) that can be suspended in a solvent of your choice.
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