97875

Atto 647

BioReagent, suitable for fluorescence, ≥90% (HPCE)

• UNSPSC Code: 12352108
• NACRES: NA.32
• MDL number: MFCD05865408

Properties

• product line: BioReagent
• Quality Segment: 100
• assay: ≥90% (HPCE)
• form: powder
• manufacturer/tradename: ATTO-TEC GmbH
• transmittance: 254 nm, 647 nm
• fluorescence: λ_ex 643 nm; λ_em 665 nm in 0.1 M phosphate pH 7.0
• λ: in ethanol (with 0.1% trifluoroacetic acid)
• UV absorption: λ: 645-651 nm A_max
• suitability: suitable for fluorescence
• storage temp.: −20°C

Description

General description

Atto 647 is a zwitterionic dye with a net electrical charge of zero. It is a pH sensitive product. While practically stable up to pH 8.5, it slowly degrades at higher pH. Atto 647 is a new label with high molecular absorption (120,000) and quantum yield (0.20) as well as enough Stokes shift between excitation and emission maximum. Atto 647 is characterized by a high photostability and brightness. In addition, this label has good water solubility and low triplet formation ability.

Application

Atto fluorescent labels are designed for high sensitivity applications, including single molecule detection. Atto labels have rigid structures that do not show any cis-trans-isomerization. Thus these labels display exceptional intensity with minimal spectral shift on conjugation.

Safety Information

• Storage Class: 11 - Combustible Solids
• wgk: WGK 3
• flash_point_f: Not applicable
• flash_point_c: Not applicable
• ppe: Eyeshields, Gloves, type N95 (US)