This product is a complex that has an estimated molecular weight of 422.5 g/mole. The solubility at 400 mM has not been tested. This product is offered in a prepared solution under product 94742 at a concentration of 200 mM. Based on the estimated molecular weight, a 0.4 M (400 mM) solution would have a concentration of 169 mg/mL. Solutions may be prepared in nuclease-free PBS containing 15% glycerol or 5% DMSO as a cryoprotectant. Solutions may then be stored in working aliquots at −20˚C. Avoid the use of EDTA in all buffer solutions as this will cause degradation of the complex. See the link below to publication that may be helpful. http://diyhpl.us/~bryan/irc/protocol-online/protocol-cache/view_cache.cgi%3FID=3486
94740
Vanadyl ribonucleoside complexes
BioReagent, for molecular biology
Synonym(s):
Ribonucleoside vanadyl complexes, VRC
Sign Into View Organizational & Contract Pricing
Select a Size
250 MG
CZK 3,830.00
1 G
CZK 12,200.00
CZK 3,830.00
Please contact Customer Service for Availability
All Photos(1)
Select a Size
Change View
250 MG
CZK 3,830.00
1 G
CZK 12,200.00
About This Item
UNSPSC Code:
12352200
NACRES:
NA.52
CZK 3,830.00
Please contact Customer Service for Availability
Recommended Products
grade
for molecular biology
Quality Level
product line
BioReagent
form
powder
suitability
in accordance for ribonuclease inhibition test
shipped in
wet ice
storage temp.
−20°C
General description
Vanadyl ribonucleoside complexes are low molecular weight inhibitors of ribonucleases.[1]
Application
Vanadyl ribonucleoside complexes (VDR) are transition state analogs that bind to active sites of many RNases, inhibiting their activity. Because the RNases are not covalently modified by the complexes, VDR must be used at every stage of RNA extraction and purification. However, VDR also inhibit RNA polymerases and in vitro translation and therefore must be removed from final preparation of RNA.
related product
Storage Class Code
11 - Combustible Solids
WGK
WGK 3
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Personal Protective Equipment
dust mask type N95 (US), Eyeshields, Gloves
Choose from one of the most recent versions:
Already Own This Product?
Find documentation for the products that you have recently purchased in the Document Library.
Customers Also Viewed
Isolation of cytoplasmic RNA: ribonucleoside-vanadyl complexes.
S L Berger
Methods in enzymology, 152, 227-234 (1987-01-01)
Analysis of TMEM174 gene expression in various renal cancer types by RNA in situ hybridization
Zhang X, et al.
Oncology Letters, 8(4), 1693-1696 (2014)
R Boom et al.
Journal of clinical microbiology, 28(3), 495-503 (1990-03-01)
We have developed a simple, rapid, and reliable protocol for the small-scale purification of DNA and RNA from, e.g., human serum and urine. The method is based on the lysing and nuclease-inactivating properties of the chaotropic agent guanidinium thiocyanate together
Inhibitors of RNases
Sambrook, J. and Russell, D.W. et al.
Molecular Cloning: A Laboratory Manual, 1, 7-7 (2001)
Analysis of AC3-33 gene expression in multiple organ cancer and adjacent normal tissue by RNA in situ hybridization
Hu F, et al.
Oncology Letters, 9(6), 2795-2798 (2015)
-
Can you provide me with a protocol for preparing a solution of Vanadyl-ribonucleoside complex (94740-1G) at a concentration of 400 mM?
Can you provide me with a protocol for preparing a solution of Vanadyl-ribonucleoside complex (94740-1G) at a concentration of 400 mM?
1 answer-
Helpful?
-
-
How to make 200 mM from 94740-250MG vanadyl ribonucleoside complexes powder?
1 answer-
The method to prepare a 200mM solution of the 94740 is proprietary and involves in-process testing. However, product 94742 and R3380 are prepared 200mM solutions of Vanadyl Ribonucleoside complexes.
Helpful?
-
Active Filters
Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.
Contact Technical Service
