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94310

Uricase from Bacillus fastidiosus

lyophilized, ~9 U/mg

Synonym(s):

Urate oxidase

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Size/SKUAvailabilityPrice
5 mg
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CZK 5,910.00
25 mg
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CZK 20,800.00

About This Item

CAS Number:
9002-12-4
UNSPSC Code:
12352204
NACRES:
NA.54
PubChem Substance ID:
57653596
MDL number:
MFCD00082126
EC Number:
1.7.3.3 (BRENDA, IUBMB)
Specific activity:
~9 U/mg
Biological source:
Bacillus sp. (B. fastidiosus)
Concentration:
90—100%

CZK 5,910.00

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biological source

Bacillus sp. (B. fastidiosus)

Quality Segment

300

form

powder

quality

lyophilized

specific activity

~9 U/mg

storage condition

dry at room temperature

concentration

90—100%

color

white to faint beige

solubility

5 mg/mL, clear, colorless to very faintly yellow (in enzyme dilution buffer)

application(s)

detection

storage temp.

−20°C

SMILES string

N.OCC1OC(C(O)C1OP(O)(=O)OCC2OC(C(O)C2O)N3C=CC(=O)NC3=O)N4C=CC(=O)NC4=O

InChI

1S/C18H23N4O14P.H3N/c23-5-7-14(13(28)16(34-7)22-4-2-10(25)20-18(22)30)36-37(31,32)33-6-8-11(26)12(27)15(35-8)21-3-1-9(24)19-17(21)29;/h1-4,7-8,11-16,23,26-28H,5-6H2,(H,31,32)(H,19,24,29)(H,20,25,30);1H3

InChI key

VPXDHZMLJOJKOX-UHFFFAOYSA-N

General description

Research area: Cell signaling
Uricase is present in many organisms, such as, archaea, bacteria, and eukaryotes. The Gut microbiome is the major source of uricase, a homotetrameric enzyme containing four identical 34 kDa subunits.

Application

Uricase may be used for the determination of uric acid in biological fluids. It may be helpful in the spectrophotometric determination of purines and purine nucleosides.

Biochem/physiol Actions

Uricase catalyzes the oxidation of uric acid to 5-hydroxyisourate, which subsequently forms (S)-allantoin. It is involved in purine metabolism, urate degradation and (S)-allantoin formation [1]The absence of functional uricase in humans has significant physiological and pathophysiological implications. It has been associated with higher serum uric acid levels, leading to an increased risk of conditions related to uric acid crystallization, such as gout. Uricase has emerged as an important therapeutic target for the treatment of conditions related to hyperuricemia, such as gout.

Other Notes

1 U corresponds to the amount of enzyme which oxidizes 1 μmole uric acid at pH 9.1 and 37 °C.
For the determination of uric acid in biological fluids; In the spectrophotometric determination of purines and purine nucleosides.

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Uricase from Bacillus fastidiosus lyophilized, ~9 U/mg

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Sigma-Aldrich

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Sigma-Aldrich

A6814

α-Amylase from Bacillus sp.

Protease from Bacillus sp. liquid, ≥16 U/g

Sigma-Aldrich

P3111

Protease from Bacillus sp.

biological source

Bacillus sp. (B. fastidiosus)

biological source

Bacillus sp. (Bacillus amyloliquefaciens)

biological source

Bacillus sp.

biological source

Bacillus sp.

specific activity

~9 U/mg

specific activity

≥0.8 U/g

specific activity

≥400 units/mg protein (Lowry)

specific activity

≥16 U/g

concentration

90—100%

concentration

-

concentration

-

concentration

-

form

powder

form

liquid

form

powder

form

liquid

application(s)

detection

application(s)

-

application(s)

-

application(s)

-

storage temp.

−20°C

storage temp.

2-8°C

storage temp.

−20°C

storage temp.

2-8°C

pictograms

Health hazard
GHS08

signalword

Danger

Hazard Classifications

Eye Irrit. 2 - Repr. 1B - Resp. Sens. 1

Storage Class

6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

Regulatory Listings

Regulatory Listings are mainly provided for chemical products. Only limited information can be provided here for non-chemical products. No entry means none of the components are listed. It is the user’s obligation to ensure the safe and legal use of the product.

1303-96-4

CAS No.

1303-96-4

CAS No.

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Questions

  1. Does this enzyme fluoresce under any wavelengths?

    1 answer

    1. This product has not been tested for fluorescence, and there is no historical data to suggest that this material will autofluoresce. This enzyme is used in combination with other reagents in the determination of uric acid. Please see the link below to review a publication that may be of interest:
      https://www.mdpi.com/2227-9040/11/5/268

      Please see the link below to review a sample or lot specific Certificate of Analysis:
      https://www.sigmaaldrich.com/product/sigma/94310#product-documentation

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