Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
93620
Trypsin inhibitor from Glycine max (soybean)
lyophilized powder, ~10000 U/mg
Synonym(s):
SBTI
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| Size/SKU | Availability | Price |
|---|---|---|
250 mg | Please contact Customer Service for Availability | 1 650,00 CZK |
1 g | Please contact Customer Service for Availability | 4 970,00 CZK |
About This Item
1 650,00 CZK
Please contact Customer Service for Availability
biological source
Glycine max (soybean)
Quality Segment
form
lyophilized powder
specific activity
~10000 U/mg
mol wt
Mr ~20000
input
plant, fungi and insect sample(s)
solubility
0.1 M phosphate pH 7.6: 5 mg/mL, clear, colorless to almost colorless
shipped in
wet ice
storage temp.
−20°C
Biochem/physiol Actions
This inhibitor acts against trypsin, and chymotrypsin and plasmin to a lesser extent. It will also inhibit proteases with mechanisms similar to trypsin, plasma kallikrein and coagulation Factor X. The trypsin inhibitor will not act against metalloproteases, tissue-baseed kallikrein, acid proteases, or thio proteases. This inhibitor acts by forming a 1:1 stoichiometric complex with the protease active site, and then cleaving a single arginine-isoleucine bond on the inhibitor. The inhibition is both reversible and pH dependent.
Preparation Note
The trypsin inhibitor is soluble in water and phosphate buffers at 10 mg/mL. It is soluble in balanced salt solutions at 1 mg/mL and in serum-free media. Concentrated solutions greater than 10 mg/mL may be hazy and have a yellow to amber color. After trypsinizing cells, resuspend in 1 mL trypsin inhibitor solution at 1 mg/mL for every mL of trypsin solution used for dissociation. The cell suspension should then be centrifuged at 1000 rpm, forming a cell pellet.
Solutions can retain activity when stored short-term at 2-8° C. Solutions are stable in frozen aliquots at -20°C.
Solutions can retain activity when stored short-term at 2-8° C. Solutions are stable in frozen aliquots at -20°C.
Other Notes
For the inhibition of proteolytic activity; Use in affinity chromatography for the purification of trypsin; Prepared by the method of M. Kunitz.
1 U corresponds to the amount of inhibitor which reduces the trypsin activity by 1 BAEE-U. (1 BAEE-U is the amount of enzyme which increases the absorbance at 253 nm by 0.001 per minute at pH 7.6 and 25 °C; BAEE, Cat. No. 12880, as substrate).
One trypsin unit = A253 of 0.001 per minute with N-alpha-benzoyl-L-arginine ethyl ester (BAEE) as substrate at pH 7.6 at 25 °C.
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This Item | |||
|---|---|---|---|
| description lyophilized powder, ~10000 U/mg | description BioReagent, For use as a marker in SDS-PAGE | description Isoelectric focusing marker, pI 4.6 | description lyophilized powder |
| biological source Glycine max (soybean) | biological source Glycine max (soybean) | biological source Glycine max (soybean) | biological source Glycine max (soybean) |
| Quality Level 100 | Quality Level 200 | Quality Level 200 | Quality Level 300 |
| form lyophilized powder | form powder | form powder | form lyophilized powder |
| shipped in wet ice | shipped in ambient | shipped in ambient | shipped in ambient |
| solubility 0.1 M phosphate pH 7.6: 5 mg/mL, clear, colorless to almost colorless | solubility serum-free medium: soluble, phosphate buffer: 10 mg/mL, balanced salt solution: 1 mg/mL, concentrate: >10 mg/mL, hazy, amber-yellow, water: 10 mg/mL | solubility serum-free medium: soluble, phosphate buffer: 10 mg/mL, concentrate: >10 mg/mL, hazy, amber-yellow, balanced salt solution: 1 mg/mL, water: 10 mg/mL | solubility water: 10 mg/mL, phosphate buffer: 10 mg/mL, serum-free medium: soluble, balanced salt solution: 1 mg/mL, concentrate: >10 mg/mL, hazy, amber-yellow |
| storage temp. −20°C | storage temp. 2-8°C | storage temp. −20°C | storage temp. 2-8°C |
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Signal Word
Danger
Hazard Codes
Precautionary Statements
Hazard Classifications
Resp. Sens. 1 - Skin Sens. 1
Storage Class
11 - Combustible Solids
WGK
WGK 3
Flash Point (°F)
Not applicable
Flash Point (°C)
Not applicable
PPE (personal protective equipment)
Eyeshields, Gloves, type N95 (US)
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What is the Department of Transportation shipping information for this product?
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What is the molecular weight and amino acid sequence of trypsin inhibitor from Glycine max (soybean)?
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Soybean trypsin inhibitor is a mixture of the three types. We have not characterized our trypsin inhibitor products in terms of how much of each of the three types may be present. All three have a molecular weight of between 20,000 and 20,200 daltons, so one might use 20,100 daltons as an average.The three types vary in the amino acids at a few positions. The detailed summary of the three types (or variants) was published in the following linked reference: J. Biochem. (Japan), 98, 435-448 (1985).Note that the authors use Tia, Tib and Tic to indicate the three variants.
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