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70540

Naphthol Yellow S

suitable for microscopy

Synonym(s):

2,4-Dinitro-1-naphthol-7-sulfonic acid sodium salt, 5,7-Dinitro-8-hydroxy-2-naphthalenesulfonic acid sodium salt, Acid Yellow 1, Flavianic acid sodium salt

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25 g
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CZK 1,490.00
CZK 745.00
100 g
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CZK 4,470.00

About This Item

Empirical Formula (Hill Notation):
C10H4N2Na2O8S
CAS Number:
846-70-8
Molecular Weight:
358.19
EC Number:
212-690-2
UNSPSC Code:
12171500
NACRES:
NA.47
PubChem Substance ID:
329762555
MDL number:
MFCD00003958
Colour Index Number:
10316
Beilstein/REAXYS Number:
3839220

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form

powder

Quality Level

200

solubility

methanol: water (1:1): 0.5 g/10 mL, clear

εmax

≥275 at 423-433 nm in water, ≥≥ 250 at 387-397 nm in water

suitability

suitable for microscopy

application(s)

diagnostic assay manufacturing
hematology
histology

storage temp.

room temp

SMILES string

[Na+].[Na+].[O-]c1c(cc([N+]([O-])=O)c2ccc(cc12)S([O-])(=O)=O)[N+]([O-])=O

InChI

1S/C10H6N2O8S.2Na/c13-10-7-3-5(21(18,19)20)1-2-6(7)8(11(14)15)4-9(10)12(16)17;;/h1-4,13H,(H,18,19,20);;/q;2*+1/p-2

InChI key

CTIQLGJVGNGFEW-UHFFFAOYSA-L

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1 of 1

This Item
495472268060700
Naphthol Yellow S suitable for microscopy

Sigma-Aldrich

70540

Naphthol Yellow S

Naphthol Yellow S analytical standard

Supelco

49547

Naphthol Yellow S

Quinoline Yellow suitable for microscopy, mixture of mono- and disulfonic acid sodium salt

Sigma-Aldrich

22680

Quinoline Yellow

Nuclear Fast Red suitable for microscopy

Sigma-Aldrich

60700

Nuclear Fast Red

suitability

suitable for microscopy

suitability

-

suitability

suitable for microscopy

suitability

suitable for microscopy

solubility

methanol: water (1:1): 0.5 g/10 mL, clear

solubility

-

solubility

H2O: 0.1 g/10 mL

solubility

methanol: water (1:1): 0.01 g/10 mL, red

application(s)

diagnostic assay manufacturing
hematology
histology

application(s)

cleaning products
cosmetics
food and beverages
personal care

application(s)

diagnostic assay manufacturing
hematology
histology

application(s)

diagnostic assay manufacturing
hematology
histology

form

powder

form

-

form

powder

form

powder

storage temp.

room temp

storage temp.

-

storage temp.

room temp

storage temp.

room temp

Quality Level

200

Quality Level

100

Quality Level

200

Quality Level

100

Application

Naphthol yellow S has been used as a staining agent in fixed human embryonic stem cell-derived cardiomyocytes.[1]

Biochem/physiol Actions

Naphthol yellow S (NYS) is used as a stain for protein basic groups. It is an acidic dye and forms a NYS-protein complex at acidic pH. Combination of feulgen and NYS provides DNA to protein ratio.

Analysis Note

λmax. ∼428 nm/∼392 nm; E(1%,1 cm) ∼275-425/∼250-400 (H2O)

pictograms

Health hazardExclamation mark
GHS08,GHS07

signalword

Warning

hcodes

H317,H373

Hazard Classifications

Skin Sens. 1 - STOT RE 2

Storage Class

11 - Combustible Solids

wgk

WGK 2

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

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Certificates of Analysis (COA)

Lot/Batch Number
WE276745/1
276745/1
0000026057
0000027025

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Scholtissek C, et al.
Chemistry and Cytochemistry of Nucleic Acids and Nuclear Proteins (2012)
S Gutschmidt et al.
Histochemistry, 72(3), 467-479 (1981-01-01)
In order to quantify the amount of protein in the small intestinal brush border region at different villus sites, cryostat sections of adult rat jejunum were stained with Naphthol Yellow S, Dinitrofluorobenzene and Coomassie Brilliant Blue and the dye deposits
M S Huang et al.
Chest, 88(6), 864-869 (1985-12-01)
In order to study differences in the tumor growth and frequency of metastases between younger and older patients with lung cancer, we investigated the nuclear DNA and nuclear protein according to age by means of cytophotometry after combined staining with
F A Bignone et al.
Bollettino della Societa italiana di biologia sperimentale, 57(9), 950-955 (1981-05-15)
In the present work we studied the reversibility of the binding reaction of Naphthol Yellow S to proteins using a Testing Automatic System Leitz. We presently think that the NY-S staining procedure, can be used as a reliable quantitative method
P Skehan et al.
In vitro cellular & developmental biology : journal of the Tissue Culture Association, 21(5), 288-290 (1985-05-01)
A rapid method has been developed for measuring the cellular protein content of mono- and multilayered anchorage cultures. Fixed or air dried cultures are stained for 30 min with 0.2% Naphthol Yellow S (NYS) dissolved in 1% acetic acid. Unbound
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Global Trade Item Number

SKUGTIN
70540-100G04061833371206
70540-25G04061833415832

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