62197

Anti-Mouse IgG (whole molecule)−Atto 488 antibody produced in goat

~1 mg/mL

• Synonym(s): Atto 488 Anti-Mouse IgG, Goat Anti-Mouse IgG
• NACRES: NA.46
• UNSPSC Code: 12352203
• Conjugate: Atto 488 conjugate
• Clone: polyclonal
• Application: ICC, IF
• Citations: 28

Properties

• biological source: goat
• Quality Segment: 100
• conjugate: Atto 488 conjugate
• antibody form: affinity isolated antibody
• antibody product type: secondary antibodies
• clone: polyclonal
• form: liquid
• contains: 50% glycerol as stabilizer
• species reactivity: mouse
• concentration: ~1 mg/mL
• technique(s): immunocytochemistry: 1:500, immunofluorescence: 1:400
• fluorescence: λ_ex 500 nm; λ_em 522 nm in PBS
• shipped in: wet ice
• storage temp.: −20°C
• target post-translational modification: unmodified

Description

General description

IgG is the most abundant antibody in human serum. It is secreted by B cells andis found in blood. It consists of four polypeptide chains, made of two identical 50 kDa γ heavy (H) chains and two identical 25 kDa κ or λ light (L) chains, that is linked together by inter-chain disulfide bonds. IgG contains four isotypes IgG1, IgG2, IgG3 and IgG4.

Immunoglobulin G (IgG) is a glycoprotein antibody that regulates immune responses such as phagocytosis and is also involved in the development of autoimmune diseases. Anti-Mouse IgG (whole molecule)-Atto 488 antibody is specific for mouse IgG. The product is purified from goat anti-mouse IgG antiserum to remove all goat serum proteins, including immunoglobulins that do not specifically bind to mouse IgG. The antibody preparation is solid phase adsorbed with human serum proteins to ensure minimal cross reactivity.

Immunogen

purified mouse IgG.

Application

Anti-Mouse IgG (whole molecule)?Atto 488 antibody produced in goat has been used:

• in immunofluorescence confocal microscopic assay for p65 localization
• in immunohistochemical analysis

Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Immunofluorescence (1 paper)

Biochem/physiol Actions

IgG facilitates transplacental transport and participates in the secondary immune response. It has the longest serum half life. It might be linked to allergy. IgG initiates the classical pathway of the complement system. It neutralizes virus particles and toxins. IgG plays a key role in antibody-dependent cell-mediated cytotoxicity (ADCC).

Physical form

Solution in 0.1 M sodium phosphate, 0.1 M NaCl, pH 7.5, andcontaining 5 mM sodium azide as a preservative.

Analysis Note

unconjugated dye ≤5% of total fluorescence

Legal Information

This product is for Research use only. In case of intended commercialization, please contact the IP-holder (ATTO-TEC GmbH, Germany) for licensing.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class: 10 - Combustible liquids
• WGK: WGK 2
• Flash Point (°F): Not applicable
• Flash Point (°C): Not applicable
• PPE (personal protective equipment): Eyeshields, Gloves