56499C

Dulbecco’s Modified Eagle’s Medium/High Modified

with 4500 mg/L dextrose, with 4.0 mM L-glutamine, with 110 mg/L sodium pyruvate, without sodium bicarbonate, powder, suitable for cell culture

• Synonym(s): Dulbecco’s Modified Eagle’s Medium - high glucose, DME, DMEM
• NACRES: NA.71
• UNSPSC Code: 12352200

Properties

• Product Name: Dulbecco′s Modified Eagle′s Medium/High Modified, with 4500 mg/L dextrose, with 4.0 mM L-glutamine, with 110 mg/L sodium pyruvate, without sodium bicarbonate, dry powder, suitable for cell culture DME, DMEM, Dulbecco’s Modified Eagle’s Medium – high Glucose. Use 13.49 g/L See information sheet, 56499C @ www.sigmaaldrich.com for hydration instructions.
• description: for research or for further manufacturing use
• Quality Segment: 500
• form: powder
• technique(s): cell culture | mammalian: suitable
• components: phenol red: yes; sodium pyruvate: yes; L-glutamine: yes; glucose: high
• shipped in: ambient
• storage temp.: 2-8°C

Description

Application

Dulbecco′s Modified Eagle′s Medium (DMEM) is a modification of Basal Medium Eagle (BME) that contains four-fold concentrations of the amino acids and vitamins. The original formulation contained 1000 mg/L of glucose and was used to culture embryonic mouse cells. Since then, it has been modified in several ways to support primary cultures of mouse and chicken cells, as well as a variety of normal and transformed cells. Each of these media offers a different combination of L-glutamine and sodium pyruvate. Additionally, the glucose levels have been raised to 4500 mg/L, contributing to the name "DMEM/High".

Preparation Note

Formulated to contain 13.5 grams of powder per liter of medium.

Safety Information

• Storage Class: 11 - Combustible Solids
• Flash Point (°F): Not applicable
• Flash Point (°C): Not applicable