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51475

Yeast

lyticase substrate, powder

Synonym(s):

Dry brewer′s yeast

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Size/SKUAvailabilityPrice
500 g
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804,00 CZK
2.5 kg
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2 050,00 CZK

About This Item

UNSPSC Code:
12352204
NACRES:
NA.83
MDL number:
MFCD00132595

804,00 CZK

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Product Name

Yeast, powder, slightly brown

form

powder

Quality Segment

100

color

slightly brown

application(s)

food and beverages

storage temp.

2-8°C

General description

Yeast is an inactive edible single-celled organism widely used in the food industry for baking, winemaking, and brewing. This product is dried irrespective of enzyme activity. Yeast serves as a substrate for lyticase.

Application

Yeast has been used:
  • as a structure modifier to alter the morphology and rheology of phase-separated biopolymer gels
  • to induce hyperthermia in rats to perform antipyretic activity test
  • in the extraction and enrichment method for the metaproteomic characterization of dissolved organic matter in groundwater samples

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This Item
YSC1YBDY1625
Sigma-Aldrich

Sigma-Aldrich

51475

Yeast

Yeast from Saccharomyces cerevisiae Type I

Sigma-Aldrich

YSC1

Yeast from Saccharomyces cerevisiae

Yeast Brewers Debittered, inactive

Sigma-Aldrich

YBD

Yeast Brewers

Yeast Extract for use in microbial growth medium

Sigma-Aldrich

Y1625

Yeast Extract

description

powder, slightly brown

description

Type I

description

Debittered, inactive

description

for use in microbial growth medium

Quality Level

100

Quality Level

200

Quality Level

200

Quality Level

300

form

powder

form

powder or solid

form

powder

form

powder

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

room temp

color

slightly brown

color

-

color

-

color

-

application(s)

food and beverages

application(s)

food and beverages
microbiology

application(s)

microbiology

application(s)

agriculture

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Storage Class

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

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Questions

  1. I would like to know how to prepare the product for using it as a substrate in Lyticase activity assay: in what concentration, heat incubation? sonication?. I will appreciate any detailed information. Thank you

    1 answer

    1. The Sigma Enzymatic Assay for Lyticase utilizes Baker’s yeast as the substrate, following the protocol outlined below:
      https://www.sigmaaldrich.com/US/en/technical-documents/protocol/protein-biology/enzyme-activity-assays/enzymatic-assay-of-lyticase
      While brewer’s yeast may be a suitable alternative, it is not guaranteed to exhibit identical behavior. Applying the same protocol is a reasonable starting point; however, experimental validation is strongly recommended to ensure reliable and reproducible results.

      Also, 1U of 04963 Sigma-Aldrich Lyticase from Bacillus subtilis corresponds to the amount of enzyme which decreases the absorbance at 800 nm by 0.001 per minute at pH 7.5 and 25 °C (Yeast, Cat. No. 51475, as substrate).
      https://www.sigmaaldrich.com/US/en/product/sigma/04963#product-documentation

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