356M-1

Cytokeratin 5 & 6 (D5 & 16B4) Mouse Monoclonal Antibody

• NACRES: NA.41
• UNSPSC Code: 12352203
• Conjugate: unconjugated
• Clone: D5 & 16B4, monoclonal
• Application: IHC (p)
• Citations: 10

Properties

• biological source: mouse
• Quality Segment: 100, 500
• conjugate: unconjugated
• antibody form: culture supernatant
• antibody product type: primary antibodies
• clone: D5 & 16B4, monoclonal
• description: For In Vitro Diagnostic Use in Select Regions (See Chart)
• form: buffered aqueous solution
• species reactivity: human
• packaging: vial of 0.1 mL concentrate (356M-14), vial of 0.5 mL concentrate (356M-15), bottle of 1.0 mL predilute (356M-17), vial of 1.0 mL concentrate (356M-16), bottle of 7.0 mL predilute (356M-18)
• manufacturer/tradename: Cell Marque^®
• technique(s): immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:50-1:200
• isotype: IgG1
• control: mesothelioma
• shipped in: wet ice
• storage temp.: 2-8°C
• visualization: cytoplasmic
• Gene Information: human ... KRT5(3852)

Description

General description

Anti-CK 5 & 6 positivity is seen in 65% to 100% of epithelioid mesotheliomas, in 0% to 19% of lung adenocarcinomas, and in 22% to 80% of serous carcinoma of the ovary and peritoneum.[1][2][3] Keratin 5 & 6 expression in lung adenocarcinoma is not common, and when it occurs, the reaction is usually confined to small focal areas or scattered cells.[1][2][3][4] The most probable cause of this finding is the presence of squamous differentiation of lung adenocarcinoma. Anti-CK 5 & 6 positivity has been useful in recognizing squamous cell carcinoma of the skin.[5][6] Less than 10% of carcinomas of the breast, colon, and prostate stain positively for this marker.[4] Anti-CK 5 & 6 has also been used successfully as a myoepithelial cell marker in the prostate and breast to determine malignancy.[7][8][9][10] Anti-CK 5 & 6 is a useful marker to distinguish lung squamous cell carcinoma from lung adenocarcinoma and large cell carcinoma within a panel including antibodies against TTF-1, Napsin A, p63, SOX-2, desmocollin3, and desmoglein3.[3][8]

Physical form

Solution in Tris Buffer, pH 7.3-7.7, with 1% BSA and <0.1% Sodium Azide

Preparation Note

Download the IFU specific to your product lot and formatNote: This requires a keycode which can be found on your packaging or product label.

Analysis Note

IVD

IVD

IVD

RUO

Other Notes

Cytokeratin 5 & 6 Positive Control Slides, Product No. 356S, are available for immunohistochemistry (formalin-fixed, paraffin-embedded sections).

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Legal Information

Cell Marque is a registered trademark of Merck KGaA, Darmstadt, Germany