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32857

Wright Stain solution

according to Wright

Synonym(s):

Eosin Methylene blue solution according to Wright, Wright’s eosin methylene blue

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1 L
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2 160,00 CZK

About This Item

NACRES:
MA.02
PubChem Substance ID:
329754363
UNSPSC Code:
12171500
MDL number:
MFCD00005038

2 160,00 CZK

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form

liquid

Quality Segment

quality

according to Wright

impurities

methanol

density

0.8 g/cm3

application(s)

diagnostic assay manufacturing
hematology
histology

storage temp.

room temp

SMILES string

[K+].COC(=O)c1ccccc1C2=C3C=C(Br)C(=O)C(Br)=C3Oc4c(Br)c([O-])c(Br)cc24

InChI

1S/C21H10Br4O5.K/c1-29-21(28)9-5-3-2-4-8(9)14-10-6-12(22)17(26)15(24)19(10)30-20-11(14)7-13(23)18(27)16(20)25;/h2-7,26H,1H3;/q;+1/p-1

InChI key

NXBZXYJXNIFCRJ-UHFFFAOYSA-M

General description

Wright′s stain solution is commercially available and is generally used in hematology. The procedure of making it includes heating methylene blue with sodium bicarbonate, followed by combining the mixture with eosin. It is an alkaline solution; hence its concentration is continuously changing, owing to the progressive oxidizing action of the alkali, therefore Giemsa′s stain is added to preserve the azurophilic properties of the polychrome dyes.

Application

Wright stain solution was used during the exudate analysis of white blood cell concentration.

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This Item
W062586137545252
Wright Stain solution according to Wright

Sigma-Aldrich

32857

Wright Stain solution

Wright stain suitable for blood stain

Sigma-Aldrich

W0625

Wright stain

Wright stain certified by the BSC

Sigma-Aldrich

861375

Wright stain

Wright stain BioReagent, suitable for microscopy

Sigma-Aldrich

45252

Wright stain

description

according to Wright

description

suitable for blood stain

description

certified by the BSC

description

BioReagent, suitable for microscopy

application(s)

diagnostic assay manufacturing
hematology
histology

application(s)

diagnostic assay manufacturing
hematology
histology

application(s)

diagnostic assay manufacturing
hematology
histology

application(s)

-

form

liquid

form

powder

form

powder

form

-

storage temp.

room temp

storage temp.

room temp

storage temp.

room temp

storage temp.

-

Quality Level

200

Quality Level

200

Quality Level

200

Quality Level

100

density

0.8 g/cm3

density

-

density

-

density

-

quality

according to Wright

quality

-

quality

-

quality

-

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Signal Word

Danger

Hazard Classifications

Acute Tox. 3 Dermal - Acute Tox. 3 Inhalation - Acute Tox. 3 Oral - Eye Irrit. 2 - Flam. Liq. 2 - Skin Sens. 1 - STOT SE 1

Target Organs

Eyes,Central nervous system

Storage Class

3 - Flammable liquids

WGK

WGK 3

Flash Point (°F)

51.8 °F

Flash Point (°C)

11 °C

PPE (personal protective equipment)

Eyeshields, Faceshields, Gloves, type ABEK (EN14387) respirator filter

Regulatory Listings

Regulatory Listings are mainly provided for chemical products. Only limited information can be provided here for non-chemical products. No entry means none of the components are listed. It is the user’s obligation to ensure the safe and legal use of the product.

67-56-1

CAS No.

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Questions

  1. Can this stain be used for analysis of WBC in bronchoalveolar lavage fluid? And what protocol to use for that? 

    1 answer

    1. This product is not specifically tested for use in this application. It would be up to the end user to determine suitability. Below find a link to a reference that may be helpful, as well as a link to the general protocol for this product.

      Wright Stain in BAL:
      https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4019586/

      Wright Stain Protocol
      https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/236/081/ws.pdf

      Helpful?

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