321R-2

PLAP (EP194) Rabbit Monoclonal Primary Antibody

• Synonym(s): Placental Alkaline Phosphatase
• UNSPSC Code: 12352203
• Conjugate: unconjugated
• Clone: EP194, monoclonal
• Application: IHC (p)
• Citations: 7

Properties

• biological source: rabbit
• conjugate: unconjugated
• antibody form: culture supernatant
• antibody product type: primary antibodies
• clone: EP194, monoclonal
• description: For In Vitro Diagnostic Use in Select Regions
• form: buffered aqueous solution
• species reactivity: human
• packaging: vial of 0.1 mL concentrate (321R-24), vial of 0.1 mL concentrate Research Use Only (321R-24-RUO), vial of 0.5 mL concentrate (321R-25), vial of 1.0 mL concentrate (321R-26), vial of 1.0 mL concentrate Research Use Only (321R-26-RUO), vial of 1.0 mL pre-dilute Research Use Only (321R-27-RUO), vial of 1.0 mL pre-dilute ready-to-use (321R-27), vial of 7.0 mL pre-dilute ready-to-use (321R-28), vial of 7.0 mL pre-dilute ready-to-use Research Use Only (321R-28-RUO)
• manufacturer/tradename: Cell Marque^®
• technique(s): immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:25-1:100 (concentrated)
• isotype: IgG
• control: placenta
• shipped in: wet ice
• storage temp.: 2-8°C
• visualization: cytoplasmic
• Gene Information: human ... ALPP(250)

Description

General description

Placental alkaline phosphatase (PLAP) is normally produced by primordial germ cells and syncytiotrophoblasts, and the detection of its expression has been useful in identifying germ cell tumors.[1] Anti-PLAP antibody immunoreacts with germ cell tumors and can discriminate between these and other neoplasms. Somatic neoplasms e.g. breast, gastrointestinal, prostatic and urinary cancers may also immunoreact with antibodies to PLAP. Anti-PLAP positivity in conjunction with keratin negativity favors seminoma over carcinoma. Germ cell tumors are usually keratin positive, but they regularly fail to stain with anti-EMA, whereas most carcinomas stain with anti-EMA.[2][3][4][5][6][7] Anti-PLAP has been useful in the diagnosis of gestational trophoblastic disease. Complete hydatidiform mole shows strong expression of hCG and weak expression of PLAP. Weak hCG and strong PLAP expression is found in partial hydatidiform mole. Choriocarcinoma presents strong expression of hCG and weak expression of hPL and PLAP.8 In addition to its role as a germ cell marker, PLAP may also be used as a myogenic marker in identifying soft tissue tumors.[1]

Physical form

Solution in Tris Buffer, pH 7.3-7.7, with 1% BSA and <0.1% Sodium Azide.

Preparation Note

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Analysis Note

IVD

IVD

IVD

RUO

Other Notes

For Technical Service please contact: 800-665-7284 or email: [email protected]

PLAP Positive Control Slides, Product No. 321S, are available for immunohistochemistry (formalin-fixed, paraffin-embedded sections).

Legal Information

Cell Marque is a registered trademark of Merck KGaA, Darmstadt, Germany

Safety Information

• Storage Class: 12 - Non Combustible Liquids
• wgk: WGK 2
• flash_point_f: Not applicable
• flash_point_c: Not applicable