This compound is tested for solubility at 10 mg/mL in Dimethylformamide. Methanol and acetonitrile are also listed as solvents, but a rate has not been established. Please see the link below to review the product datasheet:
https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/425/192/30693dat.pdf
30693
Chromeo™ P503
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| Pack Size | SKU | Availability | Price |
|---|---|---|---|
| 1 mg | Please contact Customer Service for Availability | CZK 17,100.00 |
About This Item
NACRES:
NA.32
UNSPSC Code:
12352108
Quality Level
form
lyophilized powder, solid
color
blue to very dark blue
solubility
DMF: 1 mg/mL, clear, blue
fluorescence
λex 503 nm; λem 610 nm±20 nm in 0.1 M bicarbonate buffer pH 8.3
storage temp.
−20°C
General description
Chromeo P503 labels proteins and peptides by exhibiting a color change from blue to red upon binding to primary amines. Chromeo P503 displays a weak fluorescence with a quantum yield <1% in solution. After conjugation to a primary amine group, the label undergoes a shortwave spectral shift of >100 nm and the quantum yield rises to 50%. This property allows a distinct detection of primary amines, proteins, and other biomolecules.
Application
Chromeo P503 is used as a fluorogenic reagent to label primary amine groups within molecules such as proteins. Chromeo P503 labeled proteins may be studied in a variety of gel electrophoresis and chromatographic applications.
Legal Information
Chromeo is a trademark of Active Motif Chromeon GmbH
Disclaimer
To ensure stability, the lyophilized dye should be stored at 4°C in the dark. This product is guaranteed for 6 months from the date of arrival.
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This Item | |||
|---|---|---|---|
| fluorescence λex 503 nm; λem 610 nm±20 nm in 0.1 M bicarbonate buffer pH 8.3 | fluorescence - | fluorescence - | fluorescence λex 360, 405 or 407 nm (Flow cytometry laser line), λex 404 nm; λem 431 nm, λex 405 nm (Microscopy laser line) |
| color blue to very dark blue | color - | color red to brown | color - |
| solubility DMF: 1 mg/mL, clear, blue | solubility water: 0.77 g/L at 100 °C | solubility water: 1 mg/mL, opaque, dark red | solubility DMF: soluble, DMSO: soluble, H2O: soluble |
| form lyophilized powder, solid | form powder | form powder | form solid |
| Quality Level 100 | Quality Level 200 | Quality Level 200 | Quality Level 100 |
| storage temp. −20°C | storage temp. room temp | storage temp. room temp | storage temp. −20°C |
Storage Class
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
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Related Content
Jane A Dickerson et al.
Electrophoresis, 31(15), 2650-2654 (2010-07-07)
CIEF and CZE are coupled with LIF detection to create an ultrasensitive 2-D separation method for proteins. In this method, two capillaries are joined through a buffer-filled interface. Separate power supplies control the potential at the injection end of the
Emily H Turner et al.
Journal of chromatography. A, 1194(2), 253-256 (2008-05-17)
The spectroscopic and electrophoretic properties of proteins labeled with Chromeo P503 were investigated. Its photobleaching characteristics were determined by continually infusing Chromeo P503-labeled alpha-lactalbumin into a sheath-flow cuvette and monitored fluorescence as a function of laser power. The labeled protein
Stephanie de Jong et al.
Analytical chemistry, 83(16), 6330-6335 (2011-07-07)
Methods of kinetic capillary electrophoresis (KCE) facilitate highly efficient selection of DNA aptamers for protein targets. The inability to detect native proteins at low concentrations in capillary electrophoresis creates, however, a significant obstacle for many important protein targets. Here we
Global Trade Item Number
| SKU | GTIN |
|---|---|
| 30693-1MG | 04061823353618 |
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What is the solubility of this product in Methanol?
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