14479

Phalloidin-Atto 490LS

suitable for fluorescence

• Synonym(s): Atto 490LS-Phalloidin
• NACRES: NA.32
• UNSPSC Code: 12352108

Properties

• assay: ≥75.0% (HPLC)
• Quality Segment: 100
• mol wt: M_w 1466 g/mol
• manufacturer/tradename: ATTO-TEC GmbH
• λ: in acetonitrile: water (1:1)
• UV absorption: λ: 495-501 nm A_max
• suitability: suitable for fluorescence
• storage temp.: −20°C

Description

General description

Atto 490LS is a new fluorescent label featuring an extraordinary large Stokes-Shift of 165 nm. Thus the emission spectrum is almost completely separated from its absorption spectrum, making the dye highly suitable for multiplexing experiments, in particular in combination with Atto 488 and Atto 514. Atto 490LS is very hydrophillic and shows excellent water solubility. The dye exhibits a relatively high fluorescence quantum yield, which is only slightly reduced after conjugation to biomolecules, e.g. proteins, even at high degrees of labeling (DOL). Atto 490LS is an anionic dye. After conjugation to a substrate the dye carries a net electrical charge of ^-1.
Phalloidin, a bicyclic heptapeptide, is a very strong binding reagent to actin. Fluorescent labeled phalloidin has become a useful tool to investigate the distribution of F-actin within the cytoskeleton of cells by fluorescence microscopy.

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Legal Information

This product is for Research use only. In case of intended commercialization, please contact the IP-holder (ATTO-TEC GmbH, Germany) for licensing.

Safety Information

• Storage Class: 11 - Combustible Solids
• WGK: WGK 3
• Flash Point (°F): Not applicable
• Flash Point (°C): Not applicable