11012009

ESO26

NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA.

• Synonym(s): ESO-26 Cells
• UNSPSC Code: 41106514
• Biological source: human esophagus
• Relevant disease(s): metastasis
• Growth mode: Suspension
• Morphology: Large spherical clumps of cells loosely adherent to the plastic
• Receptors: Not specified
• Technique(s): cell culture | mammalian: suitable

Properties

• biological source: human esophagus
• Quality Segment: 100
• packaging: tube of 5 μg 11012009-DNA-5UG, pkg of vial of cells 11012009-1VL
• growth mode: Suspension
• karyotype: Not specified
• morphology: Large spherical clumps of cells loosely adherent to the plastic
• products: P53 mutation c.742C>T
• receptors: Not specified
• technique(s): cell culture | mammalian: suitable
• relevant disease(s): metastasis

Description

Biochem/physiol Actions

Adenocarcinoma of the gastroesophageal junction

ESO26 was established from a primary tumour located in the gastroesophageal junction and distal oesophagus of a 56 year-old Caucasian male in 2000 involving lymph nodes and distant metastases. ESO26 was found to be tumourigenic in female NMRI nude mice or NOD-SCID mice.

STR-PCR Data:

Amelogenin: X,Y
CSF1PO: 9,10
D13S317: 9,11
D16S539: 9
D5S818: 12
D7S820: 11
THO1: 9
TPOX: 7,9
vWA: 14,18

Packaging

NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA.

Preparation Note

Maintain cultures between 2-9 x 10⁵ cells/cm³. Cells are loosely adherent and can be dislodged by gentle tapping or use of 0.02% EDTA solution. 5% CO₂; 37 °C.

RPMI-1640 + 2 mM Glutamine + 10% Foetal Bovine Serum (FBS)

Other Notes

Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.

Cultures from HPA Culture Collections and supplied by Sigma are for research purposes only. Enquiries regarding the commercial use of a cell line are referred to the depositor of the cell line. Some cell lines have additional special release conditions such as the requirement for a material transfer agreement to be completed by the potential recipient prior to the supply of the cell line. Please view the Terms & Conditions of Supply for more information.

This cell line is part of the European Collection of Authenticated Cell Cultures (ECACC), an international repository managed by the United Kingdom Health Security Agency (UKHSA). No licensing agreement is required when either this cell line or the DNA extracted from it are used for internal research purposes only. Any other use of these products is prohibited without the express written permission of UKHSA. Inquiries regarding authorized use of this cell line or its genomic DNA may be directed to culturecollections@ukhsa.gov.uk.

Disclaimer

RESEARCH USE ONLY. This product is regulated in France when intended to be used for scientific purposes, including for import and export activities (Article L 1211-1 paragraph 2 of the Public Health Code). The purchaser (i.e. enduser) is required to obtain an import authorization from the France Ministry of Research referred in the Article L1245-5-1 II. of Public Health Code. By ordering this product, you are confirming that you have obtained the proper import authorization.

Safety Information

• Storage Class: 10 - Combustible liquids
• wgk: WGK 3
• flash_point_f: Not applicable
• flash_point_c: Not applicable