11012005

OACP4 C

NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA, human gastric cardia, pleiomorphic: epithelial and fibroblastoid cells, cytoplasmic protrusions

• UNSPSC Code: 41106514
• Biological source: human gastric cardia
• Growth mode: adherent
• Karyotype: Aneuploid 53-57 chromosomes
• Morphology: Pleiomorphic: both epithelial and fibroblastoid cells with cytoplasmic protrusions
• Manufacturer/tradename: ECACC

Properties

• biological source: human gastric cardia
• Quality Segment: 100
• manufacturer/tradename: ECACC
• packaging: tube of 5 μg 11012005-DNA-5UG, pkg of vial of cells 11012005-1VL
• growth mode: adherent
• karyotype: Aneuploid 53-57 chromosomes
• morphology: Pleiomorphic: both epithelial and fibroblastoid cells with cytoplasmic protrusions
• technique(s): cell culture | mammalian: suitable

Description

Biochem/physiol Actions

OACP4 C was established from a primary tumour located in gastric cardia of a 55 year-old Caucasian male. The patient showed lymph node involvement with pleural metastases. OACP4 C was found to be tumourigenic in NMRI (female nude mice or NOD-SCID mice. The Y chromosome could not be detected in this cell line by short tandem repeat (STR)-PCR analysis when tested at ECACC. It is a known phenomenon that due to the increased genetic instability of cancer cell lines the Y chromosome can be rearranged or lost resulting in lack of detection. The cell line is identical to the source provided by the depositor based on the STR-PCR analysis.
Cultures derived from ECACC stocks of this cell line have been whole genome sequenced (Contino, et al 2016) confirming the presence of many of the known mutations that drive oesophageal cancer.

STR-PCR Data:
Amelogenin: X
CSF1PO: 11
D13S317: 12
D16S539: 12
D5S818: 9
D7S820: 9,11
THO1: 9
TPOX: 11
vWA: 16

Packaging

NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA.

Preparation Note

RPMI 1640 (R0883) + 2 mM L-Glutamine (G7513) + 10% FBS (F2442)

Split sub-confluent cultures (70-80%) up to 1:5 i.e. seeding at 1-3 x 10,000 cells/cm² using 0.25% trypsin or trypsin/EDTA; 5% CO₂; 37°C.

Other Notes

Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.

Cultures from PHE Culture Collections and supplied by Sigma are for research purposes only. Enquiries regarding the commercial use of a cell line are referred to the depositor of the cell line. Some cell lines have additional special release conditions such as the requirement for a material transfer agreement to be completed by the potential recipient prior to the supply of the cell line. Please view the Terms & Conditions of Supply for more information.

If use of this culture results in a scientific publication, it should be cited in the publication as: OACP4 C (ECACC 11012005)

This cell line is part of the European Collection of Authenticated Cell Cultures (ECACC), an international repository managed by the United Kingdom Health Security Agency (UKHSA). No licensing agreement is required when either this cell line or the DNA extracted from it are used for internal research purposes only. Any other use of these products is prohibited without the express written permission of UKHSA. Inquiries regarding authorized use of this cell line or its genomic DNA may be directed to culturecollections@ukhsa.gov.uk.

Disclaimer

RESEARCH USE ONLY. This product is regulated in France when intended to be used for scientific purposes, including for import and export activities (Article L 1211-1 paragraph 2 of the Public Health Code). The purchaser (i.e. enduser) is required to obtain an import authorization from the France Ministry of Research referred in the Article L1245-5-1 II. of Public Health Code. By ordering this product, you are confirming that you have obtained the proper import authorization.

Safety Information

• Storage Class: 10 - Combustible liquids
• wgk: WGK 3
• flash_point_f: Not applicable
• flash_point_c: Not applicable