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108315

TRIS hydrochloride

EMPROVE® EVOLVE

Synonym(s):

Trizma® hydrochloride, TRIS HCl, TRIS hydrochloride, Tris(hydroxymethyl)aminomethane hydrochloride, Tromethane hydrochloride

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Size/SKUAvailabilityPrice
100 g
Please contact Customer Service for Availability
4 450,00 CZK
300 g
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17 500,00 CZK
5 kg
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27 800,00 CZK
25 kg
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121 500,00 CZK
50 kg
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193 300,00 CZK

About This Item

Linear Formula:
NH2C(CH2OH)3 · HCl
CAS Number:
Molecular Weight:
157.60
UNSPSC Code:
12161700
Beilstein/REAXYS Number:
3675235
MDL number:

4 450,00 CZK


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biological source

synthetic

Quality Segment

description

Manufactured under appropriate controls for use as a raw material in BioProcessing

product line

EMPROVE® EVOLVE

form

powder

technique(s)

cell culture | mammalian: suitable, pH adjustment: suitable

impurities

Endotoxin, tested, Microbial, tested, Trace Metals, tested

useful pH range

7.0-9.0

pKa (25 °C)

8.1

suitability

suitable for manufacturing use

application(s)

downstream processes
pharma/biopharma processes

foreign activity

Cytotoxicity, contains (tested), DNase, contains (tested), NICKase, contains (tested), Protease, contains (tested), RNase, contains (tested)

SMILES string

Cl.NC(CO)(CO)CO

InChI

1S/C4H11NO3.ClH/c5-4(1-6,2-7)3-8;/h6-8H,1-3,5H2;1H

InChI key

QKNYBSVHEMOAJP-UHFFFAOYSA-N

General description

Our SAFC® portfolio of high-quality raw materials for use in biopharmaceutical processing withstands strict quality control procedures plus the documentation and expertise to help our customers meet requirements as defined by the M-Clarity Program.

M-Clarity Program

As part of our EMPROVE® Program, our raw materials are offered with EMPROVE® Dossiers which provide comprehensive, up-to-date documentation to help you navigate regulatory challenges, manage risks, and improve your manufacturing processes.

Buffer quality is vital for the success of biopharmaceutical processes, because buffers are indispensable in nearly every production step.

Our broad portfolio of buffer materials manufactured under appropriate controls is tailored to your needs. Ranging from non-GMP grades for low-risk application, to IPEC-PQG GMP for higher-risk applications, we have products covering all your manufacturing needs.

Application

Tris HCl is a commonly used biological buffer. Tris/Tris HCl as a buffering system has a very broad usage in biological systems for biopharmaceutical manufacturing, cell culture, diagnostics and molecular biology. The pKa of Tris is 7.77 which corresponds closely to a physiological pH of 7.36.

Tris HCl is used as the conjugate buffer component with Tris/Tromethamine/Trizma in biopharmaceutical buffer formulations (both upstream and downstream applications), electrophoresis separations and diagnostic reagent formulations.

Other Notes

The pH values of all buffers are temperature and concentration dependent. For Tris buffers, pH increases about 0.03 unit per °C decrease in temperature, and decreases 0.03-0.05 unit per ten-fold dilution. For precise applications, use a carefully calibrated pH meter with a glass/calomel combination electrode.

Legal Information

Emprove is a registered trademark of Merck KGaA, Darmstadt, Germany
SAFC is a registered trademark of Merck KGaA, Darmstadt, Germany
Trizma is a registered trademark of Merck KGaA, Darmstadt, Germany

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This Item
T6666T5941T15760
description

EMPROVE® EVOLVE

description

pH 3.5-5.0 (0.5 M in H2O), BioXtra, ≥99.0% (titration)

description

BioPerformance Certified, suitable for cell culture, ≥99.0% (titration)

description

reagent grade, ≥99%, crystalline

biological source

synthetic

biological source

-

biological source

-

biological source

synthetic

form

powder

form

powder

form

crystalline powder

form

crystalline

impurities

Microbial, tested, Endotoxin, tested, Trace Metals, tested

impurities

Insoluble matter, passes filter test

impurities

endotoxin and total aerobic microbial count, tested, ≤0.5% water (Karl Fischer), DNAse, Exonuclease; NICKase, Endonuclease; RNAse and Protease, none detected

impurities

<0.5% water

suitability

suitable for manufacturing use

suitability

-

suitability

suitable for electrophoresis, suitable for molecular biology, suitable for immunohistochemistry

suitability

-

foreign activity

NICKase, contains (tested), Protease, contains (tested), RNase, contains (tested), DNase, contains (tested), Cytotoxicity, contains (tested)

foreign activity

-

foreign activity

-

foreign activity

-

technique(s)

cell culture | mammalian: suitable

technique(s)

-

technique(s)

protein extraction: suitable, cell culture | mammalian: suitable, electrophoresis: suitable, DNA purification: suitable

technique(s)

-


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Storage Class

11 - Combustible Solids

Flash Point (°F)

Not applicable

Flash Point (°C)

Not applicable



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