104-05A

Human Epidermal Melanocytes: HEM, adult

• Synonym(s): HEM cells
• NACRES: NA.81
• UNSPSC Code: 41106514
• Biological source: human skin (normal abdominal)
• Growth mode: Adherent
• Morphology: Epidermal
• Relevant disease(s): metabolic disorders

Properties

• biological source: human skin (normal abdominal)
• packaging: pkg of 500,000 cells
• manufacturer/tradename: Cell Applications, Inc
• growth mode: Adherent
• karyotype: 2n = 46
• morphology: Epidermal
• technique(s): cell culture | mammalian: suitable
• relevant disease(s): metabolic disorders
• shipped in: dry ice
• storage temp.: −196°C

Description

General description

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Epidermal melanocytes are pigment-producing cells located at the basal level of epidermis, where they interact with keratinocytes via cellular processes called dendrites. Melanin, the pigment produced by melanocytes and responsible for skin color, is then transferred to keratinocytes, where it is stored in vesicles called melanosomes located around the nucleus to provide protection from UV radiation. HEM maintain their characteristic shape in culture for many generations. They produce melanin and serve as a useful cell model for the studies of melanocyte proliferation and differentiation, as well as progression of melanocytic neoplasia.

Application

melanin production, melanocyte proliferation, differentiation

Biochem/physiol Actions

Skin

Preparation Note

• 2nd passage, >500,000 cells in Basal Medium containing 30% FBS & 10% DMSO
• Can be cultured at least 12 doublings

Please refer to the HEM Culture Protocol.

Other Notes

Basal Medium containing 30% FBS & 10% DMSO

Disclaimer

RESEARCH USE ONLY. This product is regulated in France when intended to be used for scientific purposes, including for import and export activities (Article L 1211-1 paragraph 2 of the Public Health Code). The purchaser (i.e. enduser) is required to obtain an import authorization from the France Ministry of Research referred in the Article L1245-5-1 II. of Public Health Code. By ordering this product, you are confirming that you have obtained the proper import authorization.

Safety Information

• Storage Class: 11 - Combustible Solids
• wgk: WGK 3
• flash_point_f: Not applicable
• flash_point_c: Not applicable