10072802

CCB cell line from carp

NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA.

• NACRES: NA.81
• UNSPSC Code: 41106514
• Biological source: fish brain
• Growth mode: Adherent
• Morphology: Squamous
• Receptors: Not specified
• Technique(s): cell culture | mammalian: suitable

Properties

• biological source: fish brain
• Quality Segment: 100
• manufacturer/tradename: ECACC
• packaging: tube of 5 μg 10072802-DNA-5UG, pkg of vial of cells 10072802-1VL
• growth mode: Adherent
• karyotype: Not specified
• morphology: Squamous
• products: Not specified
• receptors: Not specified
• technique(s): cell culture | mammalian: suitable

Description

Biochem/physiol Actions

Common carp brain cell line, koi herpesvirus isolation

The CCB cell line was developed from Common carp (Cyprinus carpio carpio) brain.

Packaging

NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA.

Preparation Note

EMEM (EBSS) + 2 mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 10% Fetal Bovine Serum (FBS). 10 mM HEPES can be included if a CO₂ incubator is not available.

Split sub-confluent cultures (70-80%) 1:4 to 1:6, i.e., seeding at 2 x 10⁴ cells/cm₂ using 0.25% trypsin or trypsin/EDTA; 5% CO₂; 25 °C.

Other Notes

Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.

Cultures from HPA Culture Collections and supplied by Sigma are for research purposes only. Enquiries regarding the commercial use of a cell line are referred to the depositor of the cell line. Some cell lines have additional special release conditions such as the requirement for a material transfer agreement to be completed by the potential recipient prior to the supply of the cell line. Please view the Terms & Conditions of Supply for more information.

This cell line is part of the European Collection of Authenticated Cell Cultures (ECACC), an international repository managed by the United Kingdom Health Security Agency (UKHSA). No licensing agreement is required when either this cell line or the DNA extracted from it are used for internal research purposes only. Any other use of these products is prohibited without the express written permission of UKHSA. Inquiries regarding authorized use of this cell line or its genomic DNA may be directed to culturecollections@ukhsa.gov.uk.

Safety Information

• Storage Class: 10 - Combustible liquids
• WGK: WGK 3
• Flash Point (°F): Not applicable
• Flash Point (°C): Not applicable