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Merck

07679

Abberior® STAR 635P, NHS ester

suitable for fluorescence

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1 MG

CZK 19,900.00

CZK 19,900.00


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About This Item

NACRES:
NA.32
UNSPSC Code:
12352108

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mol wt

Mw 1030 g/mol

solubility

DMF: 0.25 mg/mL, clear

fluorescence

λex 634 nm; λem 654 nm±10 nm in PBS, pH 7.4

suitability

suitable for fluorescence

storage temp.

−20°C

Quality Level

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This Item
383774335418373
suitability

suitable for fluorescence

suitability

-

suitability

-

suitability

suitable for fluorescence

fluorescence

λex 634 nm; λem 654 nm±10 nm in PBS, pH 7.4

fluorescence

λex 583 nm; λem 605.0 nm±10 nm in PBS, pH 7

fluorescence

λex 637 nm; λem 660 nm±10 nm in water

fluorescence

-

solubility

DMF: 0.25 mg/mL, clear

solubility

DMF: 1 mg/mL, clear

solubility

-

solubility

-

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−20°C

Quality Level

100

Quality Level

100

Quality Level

100

Quality Level

100

mol wt

Mw 1030 g/mol

mol wt

-

mol wt

-

mol wt

-

General description

Abberior STAR635P is the first member of an entirely new class of dyes introduced exclusively by Abberior - first commercially available phosphorylated fluorescent dye. The dye excels in its photophysical parameters, e.g. very bright, very stable and it enables basically background free imaging.
Abberior STAR 635P can substitute Atto 647N, AlexaFluor 647, or Cy5. It can be excited with diode lasers (635 nm, 650 nm) or with the 647 nm line of a Krypton laser. For STED, a depletion wavelength of 750 - 780 nm is recommended. Please see reference1 for detailled characteristics.
Best results are obtained with freshly prepared samples.

Abberior STAR 635P particularly well suits the Leica TCS STED system and delivers high-resolution STED images with unmatched quality, e.g. extremely low/no background. The dye serves as an ideal partner for several 2-color STED.

Key Features
  • First phosphorylated dye
  • Unmatched, background free STED imaging contrast
  • Verified in the Leica STED system

Absorption Maximum, λmax: 638 nm (PBS, pH 7.4)
Extinction Coefficient, ε(λmax): 120′000 M-1cm-1 (PBS, pH 7.4)
Correction Factor, CF260 = ε260/εmax: 0.21 (PBS, pH 7.4)
Correction Factor, CF280 = ε280/εmax: 0.40 (PBS, pH 7.4)
Fluorescence Maximum, λfl: 651 nm (PBS, pH 7.4)
Recommended STED Wavelength, λSTED: 750 - 780 nm
Fluorescence Quantum Yield, η: 0.90 (PBS, pH 7.4)
Fluorescence Lifetime, τ: 3.3 ns (PBS, pH 7.4)

Application

Designed and tested for fluorescent super-resolution microscopy

Legal Information

Atto is a trademark of Atto-Tec GmbH
abberior is a registered trademark of Abberior GmbH

related product

Storage Class

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable


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Sören Brandenburg et al.
Frontiers in physiology, 9, 1227-1227 (2018-10-24)
Rationale: Recently, abundant axial tubule (AT) membrane structures were identified deep inside atrial myocytes (AMs). Upon excitation, ATs rapidly activate intracellular Ca2+ release and sarcomeric contraction through extensive AT junctions, a cell-specific atrial mechanism. While AT junctions with the sarcoplasmic

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