![7-Diethylamino-3-[N-(2-maleimidoethyl)carbamoyl]coumarin BioReagent, suitable for fluorescence, ≥97.0% (HPLC)](/deepweb/assets/sigmaaldrich/product/structures/341/038/a7e0c464-8abe-4eb0-b253-6537f649d89c/640/a7e0c464-8abe-4eb0-b253-6537f649d89c.png)
The product specification requires an emission wavelength range of 460-470nm. The product is not screened for excitation wavelength, however it is expected to fall within a range of 416 to 425 nm. The compound is typically used for real-time measurements for the release of inorganic phosphates during enzymatic reaction when MDCC is conjugated to a mutant phosphate-binding protein . Please see the link below to review a publication that may be of interest:
https://pubs.acs.org/doi/10.1021/bi9804277
05019
7-Diethylamino-3-[N-(2-maleimidoethyl)carbamoyl]coumarin
BioReagent, suitable for fluorescence, ≥97.0% (HPLC)
Synonym(s):
MDCC
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| Size/SKU | Availability | Price |
|---|---|---|
5 mg | Please contact Customer Service for Availability | CZK 4,570.00 |
About This Item
Empirical Formula (Hill Notation):
C20H21N3O5
CAS Number:
Molecular Weight:
383.40
UNSPSC Code:
12352116
PubChem Substance ID:
NACRES:
NA.32
MDL number:
product line
BioReagent
assay
≥97.0% (HPLC)
suitability
suitable for fluorescence
SMILES string
CCN(CC)c1ccc2C=C(C(=O)NCCN3C(=O)C=CC3=O)C(=O)Oc2c1
InChI
1S/C20H21N3O5/c1-3-22(4-2)14-6-5-13-11-15(20(27)28-16(13)12-14)19(26)21-9-10-23-17(24)7-8-18(23)25/h5-8,11-12H,3-4,9-10H2,1-2H3,(H,21,26)
InChI key
IXQPRUQVJIJUEB-UHFFFAOYSA-N
Application
Thiol-reactive probe for protein labelling
7-Diethylamino-3-[N-(2-maleimidoethyl)carbamoyl]coumarin is utilized as a fluoresencent biological sensing device . Used for real-time measurements for the release of inorganic phosphates during enzymatic reaction when MDCC is conjugated to a mutant phosphate-binding protein . Also, utilized for intramolecular fluorescence energy transfer (FRET) experiments .
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| suitability suitable for fluorescence | suitability - | suitability - | suitability - |
| assay ≥97.0% (HPLC) | assay ≥90% | assay - | assay - |
| Quality Level 100 | Quality Level 200 | Quality Level 100 | Quality Level 200 |
| product line BioReagent | product line - | product line - | product line - |
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