UPTQBF2

Ultra pure 10x Taq Buffer without MgCl2

DNA-free, Nuclease-free 10x PCR reaction buffer without MgCl2, designed for further manufacture

• UNSPSC Code: 41106229
• NACRES: NA.25

Properties

• Quality Segment: 400
• form: liquid
• feature: DNA free, RNase and DNase free (also Nickase free)
• packaging: pkg of 1.5 mL
• technique(s): RT-PCR: suitable, cDNA synthesis: suitable
• shipped in: dry ice
• storage temp.: −20°C

Description

General description

Ultra pure 10x Taq Buffer without MgCl2 is manufactured under stringent conditions to ensure that it is free of detectable levels of DNA, DNase, RNase, and Nickase to reduce the risk of false positive and false negative results in molecular diagnostic assays. When used with Ultra pure 25mM MgCl2, Ultra pure 10x Taq Buffer without MgCl2 allows flexibility to optimize amplification conditions through adjustments to MgCl2 concentration. Ultra pure 10x Taq Buffer without MgCl2 should be used with other ultra pure DNA-free reagents, such as Ultra pure JumpStart^™ Taq DNA Polymerase and Ultra pure DNA-free Water, to minimize false positive and negative results and improved DNA amplification.
Ultra pure 10x Taq Buffer without MgCl2 was designed for use in highly-regulated industries in applications that require a high level of performance, lot-to-lot consistency, and high sensitivity without false results from reagent-borne contamination while meeting industry-driven critical regulations. To meet this requirements we have increased the quality level of our products. The M-Clarity Program defines product quality. It provides transparency so that you can choose, with confidence, suitable products that meet your needs with respect to quality and regulatory compliance, transparency, change notification, and documentation.

Application

Ultra pure 10x Taq Buffer without MgCl2 can be used for:

• Quantitative PCR
• Amplification of complex genomic DNA and cDNA templates
• Amplification of low-copy number targets
• Microbiome analysis
• For further manufacture

Features and Benefits

• DNA-free
• DNase-free, RNase-free, and nickase-free
• Ideal for high throughput quantitative PCR applications
• Compatible with Ultra pure JumpStart^™ Taq DNA Polymerase
• Flexibility to optimize amplification conditions
• Enhanced change control and quality agreement

Packaging

Ultra pure 10x Taq Buffer without MgCl2 is provided in larger SKU sizes for ease of use in high throughput PCR applications and for further manufacture. Ultra pure 10x Taq Buffer without MgCl2 should be used with other ultra pure DNA-free reagents, such as Ultra pure JumpStart^™ Taq DNA Polymerase, Ultra pure 25mM MgCl2, and Ultra pure DNA-free Water, to minimize false positive and negative results.

Other Notes

Why Choose Our Ultra pure & DNA-Free 10x Taq Buffer without MgCl₂?

• Full Control Over MgCl₂ Concentration

• - Unlike pre-formulated buffers, our 10x Taq Buffer without MgCl₂ allows you to fine-tune MgCl₂ concentration independently using Ultra Pure 25 mM MgCl₂ (UPMGCL2). Since MgCl₂ concentration directly influences primer annealing temperature, polymerase fidelity, specificity, and product yield, this flexibility is critical for optimizing challenging or sensitive assays.
• Ultra pure & DNA-free- Manufactured under stringent quality controls, this buffer is certified free of detectable levels of DNA, DNase, RNase, and Nickase.
• Designed for Regulated Industries- Developed specifically for highly regulated industries, this buffer delivers the lot-to-lot consistency, sensitivity, and reliability required for molecular diagnostics, clinical research, and further manufacture. Each batch is manufactured and tested to meet stringent industry-driven standards.
• M-Clarity Program- Our Ultra pure 10x Taq Buffer without MgCl₂ is supported by the M-Clarity Program, which provides full transparency across quality standards, regulatory compliance documentation, and change notification protocols. This gives laboratories and manufacturers the confidence to integrate our products into regulated workflows with full traceability and documentation support.
• Seamless Compatibility with the Ultra pure Ecosystem This buffer is fully compatible with Ultra pure JumpStart^™ Taq DNA Polymerase (UPJSTAQ), Ultra pure 25 mM MgCl₂ (UPMGCL2), and Ultra pure DNA-Free Water (UPH2O), forming part of a complete, matched reagent system designed to minimize false positive and negative results across all PCR workflows.
• Scalable for High-Throughput & Further Manufacture- Available in larger SKU sizes, this buffer is well-suited for high-throughput quantitative PCR applications and further manufacture, providing consistency and convenience at scale without compromising on purity or performance.

Quality testing ensures the absence of detectable levels of contaminating DNA (<=1 copy of prokaryotic gDNA, <=0.1 copy of eukaryotic gDNA, and <=10 copies of plasmid DNA per 100 units of enzyme) as detected by qPCR using universal primers against 16S capable of detecting > 500,000 prokayotes, 18S capable of detecting > 110,000 eukaryotes, and Ori capable of detecting > 25,000 plasmids, respectively.

Legal Information

JumpStart is a trademark of Sigma-Aldrich Co. LLC

Safety Information

• Storage Class: 10 - Combustible liquids
• WGK: WGK 1
• Flash Point (°F): Not applicable
• Flash Point (°C): Not applicable