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S2255

Safranin O

Dye content ≥85 %, Powder

Synonym(s):

Basic Red 2, Cotton Red, Gossypimine, Safranin T, Safranin Y or A

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Size/SKUAvailabilityPrice
25 g
Please contact Customer Service for Availability
CZK 4,390.00
100 g
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CZK 8,890.00

About This Item

Empirical Formula (Hill Notation):
C20H19ClN4
CAS Number:
477-73-6
Molecular Weight:
350.84
UNSPSC Code:
12171500
NACRES:
NA.47
PubChem Substance ID:
24899516
EC Number:
207-518-8
MDL number:
MFCD00011759
Colour Index Number:
50240
Beilstein/REAXYS Number:
3924099

CZK 4,390.00

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Product Name

Safranin O, Dye content ≥85 %

form

powder

Quality Segment

200

composition

Dye content, ≥85%

technique(s)

microbe id | staining: suitable

pH

10 (20 °C, 10 g/L)

solubility

water: 1 mg/mL, clear, dark red to very dark red and red purple

εmax

1250-1650 at 530-534 nm in 50% ethanol

application(s)

diagnostic assay manufacturing
hematology
histology

storage temp.

room temp

SMILES string

[Cl-].Cc1cc2nc3cc(C)c(N)cc3[n+](-c4ccccc4)c2cc1N

InChI

1S/C20H18N4.ClH/c1-12-8-17-19(10-15(12)21)24(14-6-4-3-5-7-14)20-11-16(22)13(2)9-18(20)23-17;/h3-11H,1-2H3,(H3,21,22);1H

InChI key

OARRHUQTFTUEOS-UHFFFAOYSA-N

General description

Safranin O is a cationic dye, which displays less significant metachromasia. It is used to detect any variations in articular disease. Safranin O binds specifically to polyanions. It detects the amount of proteoglycan present in cartilages. Safranin O is used as a marker for glycosaminoglycan depletion in osteoarthritis. Safranin is used as a counter stain in Gram staining.

Application

Safranin O has been used in the histological analysis of femurs. It has also been used to detect the presence of glycosaminoglycans in the inner body of meniscus structures.

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This Item
8412094635S8884
Safranin O Dye content ≥85 %

Sigma-Aldrich

S2255

Safranin O

Safranin O suitable for microscopy

Sigma-Aldrich

84120

Safranin O

Gram’s safranin solution suitable for microscopy

Sigma-Aldrich

94635

Gram′s safranin solution

Safranin O certified by the BSC

Sigma-Aldrich

S8884

Safranin O

technique(s)

microbe id | staining: suitable

technique(s)

titration: suitable

technique(s)

microbe id | staining: suitable

technique(s)

microbe id | staining: suitable

form

powder

form

powder or crystals

form

liquid

form

powder

solubility

water: 1 mg/mL, clear, dark red to very dark red and red purple

solubility

methanol: 0.01 g/10 mL, red

solubility

water: soluble at 20 °C

solubility

H2O: 1 mg/mL

application(s)

diagnostic assay manufacturing
hematology
histology

application(s)

diagnostic assay manufacturing
hematology
histology
microbiology

application(s)

diagnostic assay manufacturing
hematology
histology

application(s)

diagnostic assay manufacturing
hematology
histology

composition

Dye content, ≥85%

composition

-

composition

ethanol, 10%

composition

Dye content, ≥80%

Quality Level

200

Quality Level

200

Quality Level

100

Quality Level

200

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pictograms

Corrosion
GHS05

signalword

Danger

hcodes

H318

Hazard Classifications

Eye Dam. 1

Storage Class

11 - Combustible Solids

wgk

WGK 3

ppe

dust mask type N95 (US), Eyeshields, Gloves

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Questions

1–2 of 2 Questions  

  1. Do you have any recommendations for the storage temperature and corresponding shelf life of Safranin O after preparation?

    1 answer

    1. As Safranin O can be prepared in a variety of solvents and at varying concentrations, it is really not possible to give precise guidelines for solution stability.

      There is a Safranin O solution that is part of a Gram Stain kit (HT90A). According to the kit Instructions For Use, this solution has 0.6% dye in 20% SD3A reagent alcohol. The expiration date for this solution is 24 months. But if the formulation varies from this exact composition or if other dyes or chemicals are also present, the 24 months expiration dating may or may not be appropriate

      Helpful?

  2. Good morning, how can i use the safranin O stain for detection of in vitro GAG ? I need the steps of preparation

    1 answer

    1. Safranin O is noted for its use in visualizing and quantifying the presence of glycosaminoglycans (GAGs) within a tissue sample. However, this application is not specifically tested, and no protocol is available to recommend. It is advised to refer to academic literature for guidance.

      Helpful?

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