66720

Methylene Blue hydrate

purum p.a., suitable for microscopy, ≥97.0% (calc. based on dry substance, AT)

• Empirical Formula (Hill Notation): C₁₆H₁₈ClN₃S · xH₂O
• CAS Number: 122965-43-9
• Molecular Weight: 319.85 (anhydrous basis)
• UNSPSC Code: 12171500
• NACRES: NA.32
• PubChem Substance ID: 329760310
• EC Number: 200-515-2
• Beilstein/REAXYS Number: 3599847
• MDL number: MFCD00150006

Properties

• grade: purum p.a.
• Quality Segment: 200
• assay: ≥97.0% (calc. based on dry substance, AT)
• form: powder
• mol wt: 319.85 g/mol (anhydrous basis)
• concentration: ≤100% (Methylthioninium chloride hydrate)
• color: green to dark green
• solubility: ethanol: 0.01 g/10 mL, blue to very deep blue
• λmax: 656-666 nm in water
• suitability: suitable for microscopy
• storage temp.: room temp
• SMILES string: O.[Cl-].CN(C)c1ccc2N=C3C=C\C(C=C3Sc2c1)=[N+](/C)C
• InChI: 1S/C16H18N3S.ClH.H2O/c1-18(2)11-5-7-13-15(9-11)20-16-10-12(19(3)4)6-8-14(16)17-13;;/h5-10H,1-4H3;1H;1H2/q+1;;/p-1
• InChI key: WQVSELLRAGBDLX-UHFFFAOYSA-M

Description

General description

Methylene Blue (MB) is a basic histological dye.

Application

Methylene Blue hydrate has been used:

• in the preparation of methylene blue (MB) solution to stain zymograms
• to label the injection site in the tissue slices
• to pre-treat mice via oral gavage to study its effects on noise-induced hearing loss

Biochem/physiol Actions

Methylene blue (MB) plays a key role in microbiology and pharmacology. It is mainly used in staining live organisms and in methemoglobinemia treatment. MB can be considered as a possible and safe vasoconstrictor. It has several applications in textile, paper and hair coloring sectors.

Other Notes

On the efficiency of methylene blue versus persulfate catalysis of polyacrylamide gels, as investigated by capillary zone electrophoresis[1]

may contain up to 22% water

Safety Information

• pictograms: GHS07
• signalword: Warning
• hcodes: H302
• Hazard Classifications: Acute Tox. 4 Oral
• Storage Class: 11 - Combustible Solids
• wgk: WGK 3
• flash_point_f: Not applicable
• flash_point_c: Not applicable
• ppe: dust mask type N95 (US), Eyeshields, Gloves