53189

Mannitol Lysine Crystal Violet Brilliant Green Agar

NutriSelect^® Plus, powder, nonselective and differential for Salmonella spp., pack of 500 g, suitable for microbiology

• Synonym(s): MLCB AGAR, Mannitol Lysine Agar
• NACRES: NA.85
• UNSPSC Code: 41171606
• Manufacturer/tradename: NutriSelect^® Plus
• Form: powder
• Suitability: nonselective and differential for Salmonella spp.
• Application(s): clinical testing; food and beverages; veterinary, microbiology

Properties

• sterility: non-sterile
• Quality Segment: 200
• form: powder
• shelf life: limited shelf life, expiry date on the label
• composition: agar, 15.0 g/L , beef extract, 2.0 g , briliant green, 0.0125 g/L , crystal violet, 0.010 g/L , ferric ammonium citrate, 1.0 g/L , L-lysine hydrochloride, 5.0 g/L , mannitol, 3.0 g/L , peptic digest of animal tissue, 10.0 g/L , sodium chloride, 4.0 g/L , sodium thiosulfate, 4.0 g/L , yeast extract, 5.0 g/L
• packaging: pack of 500 g
• manufacturer/tradename: NutriSelect^® Plus
• technique(s): microbiological culture: suitable
• final pH: 6.8±0.2 (25 °C)
• application(s): clinical testing; food and beverages; veterinary, microbiology
• suitability: nonselective and differential for Salmonella spp.

Description

General description

Mannitol Lysine Crystal Violet Brilliant Green Agar (MLCB Agar) is formulated for the selective isolation of Salmonella species, excluding Salmonella typhi and Salmonella paratyphi A, from fecal and food products. The medium contains peptic digest of animal tissue, beef extract, and yeast extract, which provides essential nutrients to support the growth of Salmonella. Mannitol serves as the fermentable carbohydrate, enabling the detection of fermentation activity, while L-lysine acts as the amino acid that facilitates lysine decarboxylation, a key metabolic process in Salmonella identification. This selective medium is designed to enhance the isolation and differentiation of Salmonella species in complex sample matrices.

Application

Mannitol Lysine Crystal Violet Brilliant Green Agar is used for isolating Salmonella species, excluding Salmonella typhi and Salmonella paratyphi A, from fecal samples and food products. It is widely applied in clinical testing for disease diagnosis, the food and beverage industry for ensuring microbiological safety, veterinary testing for disease control in animals, and microbiology research for studying Salmonella behavior in various environments.

Features and Benefits

NutriSelect^® Plus is a powdered culture media offering

• Quality control with growth promotion test according to industry standards
• Quality assurance acc. to ISO 9001
• Cost-efficient powdered media

Preparation Note

Suspend 49.02 grams in 1000 ml distilled water. Heat to boiling to dissolve the medium completely. DO NOT AUTOCLAVE. Cool to 45-50°C. Mix well and pour into sterile Petri plates.

Other Notes

Salmonellae grow as large purple-black colonies with black centers due to hydrogen sulfide production, facilitated by sodium thiosulfate and ferric ammonium citrate. Mannitol fermentation produces acid, stimulating lysine decarboxylation, which elevates the pH and promotes blackening. Atypical Salmonella strains producing little or no hydrogen sulfide may appear as mauve-grey colonies with a central black "bull′s eye." Mannitol Lysine Medium can be directly inoculated or used after enrichment in Modified Semisolid RV Medium Base. For better detection of atypical strains, Brilliant Green Agar (Modified) or Bismuth Sulfite Agar should be used alongside Mannitol Lysine Medium.

We offer two media types: the superior granulated GranuCult^® and the cost-efficient powdered NutriSelect^® culture media, depending on your needs.
The designations basic, plus, or prime are added to indicate the quality control level, from basic quality control to standard QC plus to prime for full regulatory compliance.

Legal Information

GRANUCULT is a registered trademark of Merck KGaA, Darmstadt, Germany

NutriSelect is a registered trademark of Merck KGaA, Darmstadt, Germany

Safety Information

• hcodes: H412
• pcodes: P273 - P501
• Hazard Classifications: Aquatic Chronic 3
• Storage Class: 11 - Combustible Solids
• wgk: WGK 3
• flash_point_f: Not applicable
• flash_point_c: Not applicable