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Merck

BSAV-RO

Roche

Bovine Serum Albumin

≥98.5%, New Zealand origin

Synonym(s):

Bovine Serum Albumin Fraction V

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50 G

CZK 6,080.00

100 G

CZK 11,300.00

500 G

CZK 41,100.00

1 KG

CZK 75,600.00

CZK 6,080.00


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About This Item

UNSPSC Code:
12352202
NACRES:
NA.54
Biological source:
bovine
Assay:
≥98.5%
Form:
lyophilized
Technique(s):
ELISA: suitable, Southern blotting: suitable, western blot: suitable
Impurities:
<0.0003 Lithium (Flame photometry), <0.001 Iron (bathophenanthraline), <0.002 P (inorganic), <0.003 Heavy metals (as Pb), <0.003 Magnesium (xylidyl blue), <0.005 Glycerol (enzyme), <0.006 potassium (Flame photometry), <0.02 Calcium ( o-cresolphthalein), <0.05 glucose (enzyme), <0.1 L-lactate, <0.15 chloride (mercurom), <0.5 Sodium (flame photometry), <100 microorganisms (organism/g), <5 water

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assay

≥98.5%

biological source

bovine

form

lyophilized

mol wt

Mr =68 kDa

packaging

pkg of 1 kg (10735108001), pkg of 100 g (10735086001), pkg of 50 g (10735078001), pkg of 500 g (10735094001)

manufacturer/tradename

Roche

origin

New Zealand origin

technique(s)

ELISA: suitable, Southern blotting: suitable, western blot: suitable

impurities

<0.0003 Lithium (Flame photometry), <0.001 Iron (bathophenanthraline), <0.002 P (inorganic), <0.003 Heavy metals (as Pb), <0.003 Magnesium (xylidyl blue), <0.005 Glycerol (enzyme), <0.006 potassium (Flame photometry), <0.02 Calcium ( o-cresolphthalein), <0.05 glucose (enzyme), <0.1 L-lactate, <0.15 chloride (mercurom), <0.5 Sodium (flame photometry), <100 microorganisms (organism/g), <5 water

absorption

≤0.4 at 550 nm

UniProt accession no.

foreign activity

Microorganisms <100 (Organism/g)

storage temp.

2-8°C

Gene Information

bovine ... ALB(280717)

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1 of 4

This Item
81053381053N810535
assay

≥98.5%

assay

≥98% (purity)

assay

≥98% (purity)

assay

≥98% (purity)

origin

New Zealand origin

origin

USA origin

origin

USA origin

origin

USA origin

technique(s)

ELISA: suitable, western blot: suitable, Southern blotting: suitable

technique(s)

blood typing: suitable ( )

technique(s)

blood typing: suitable ( )

technique(s)

blood typing: suitable ( )

biological source

bovine

biological source

bovine serum

biological source

bovine serum

biological source

bovine serum

Quality Level

100

Quality Level

300

Quality Level

300

Quality Level

300

form

lyophilized

form

lyophilized powder

form

lyophilized powder

form

lyophilized powder

General description

For standard applications.

Application

Bovine Serum Albumin Fraction V is suitable for standard applications such as:
  • Stabilizer to prevent proteolysis and denaturation of enzymes and other proteins, especially when the proteins are present in low concentrations.[1]
  • Buffer component in immunochemistry, biochemistry, cell biology, or molecular biology
  • Reducing agent
  • Protein blocker for saturation of protein binding sites in ELISAs, Southern blots, and western blots[2]
  • Media supplement for media that require addition of protein
  • Carrier protein
  • Standard protein in gel electrophoresis and protein determination[3]

Preparation Note

Storage conditions (working solution): In solution, albumin is stable for about three months at -15 to -25 °C.

Analysis Note

Purity: ≤95% protein, ≤5% H2O; electrophoretically homogeneous
Contaminants: <0.5% Na (flame photometric), <0.006% K (flame photometric), <0.0003% Li (flame photometric), <0.02% Ca (o-cresolphthalein), <0.003% Mg (xylidyl blue), <0.003% heavy metals (as Pb), <0.001% Fe (bathophenanthroline), <0.002% Pi, <0.05% glucose (enzymatic), <0.005% glycerol (enzymatic), <0.025% L-lactate (enzymatic)

Other Notes

For life science research only. Not for use in diagnostic procedures.

Storage Class

11 - Combustible Solids

wgk

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable


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Southwestern blotting is used to investigate DNA-protein interactions. The advantage of this technique over other related methods such as electrophoretic mobility shift assay (EMSA) and DNA footprinting is that it provides information regarding the molecular weight of unknown protein factor.
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The Journal of reproduction and development, 59(4), 346-352 (2013-04-09)
Cortisol (Cr), the most important glucocorticoid (GC), is well known to suppress uterine prostaglandin F2α (PGF) production. However, the details of the regulatory mechanisms controlling the cyclic changes in endometrial PGF production remain unclear. Here we investigated the expression of

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