11814389001

Red Blood Cell Lysis Buffer

solution, Roche, pkg of 100 mL, sufficient for 50-500 reactions

• Synonym(s): RBC Lysis Buffer, lysis buffer, red blood cells
• UNSPSC Code: 23201100

Properties

• form: solution
• usage: sufficient for 50-500 reactions
• packaging: pkg of 100 mL
• manufacturer/tradename: Roche
• storage temp.: 2-8°C

Description

General description

Used for both DNA and RNA isolation, the buffer is designed for the preferential lysis of red blood cells from human whole blood, yielding intact white blood cells (free of red blood cells) for further applications. This buffer is not intended for use with whole blood from any other species.

Application

Red blood cell lysis buffer has been used in the isolation of PBMCs (peripheral blood mononuclear cells) from HIV infected patients.[1] It has also been used in the isolation of lung cellsm,[2] naive CD8+ T cells[3] and infected cells from the lung.[4]

Used for both DNA and RNA isolation, the buffer is designed for the preferential lysis of red blood cells from human whole blood, yielding intact white blood cells (free of red blood cells) for use in further research applications.
It has been used for the lysis of blood cells in femoral bone marrow, PBMC (peripheral blood mononuclear cells) and human lung adenocarcinoma samples.[5][6][7]

Physical form

Ready-to-use solution.

Other Notes

For life science research only. Not for use in diagnostic procedures.

Safety Information

• WGK: nwg
• Flash Point(F): No data available
• Flash Point(C): No data available