11721925001
Roche
DNA Molecular Weight Marker XIII (50 bp ladder)
pkg of 50 μg (250 μg/ml), solution
Synonym(s):
DNA marker
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About This Item
UNSPSC Code:
41105335
Recommended Products
form
solution
Quality Level
packaging
pkg of 50 μg (250 μg/ml)
manufacturer/tradename
Roche
concentration
250 μg/mL
color
colorless
solubility
water: miscible
storage temp.
−20°C
General description
Fragment mixture prepared by cleavage of a specially constructed plasmid with restriction endonucleases.
Size Range: 50 to 750 bp and an additional band of 2642 bp
Size Range: 50 to 750 bp and an additional band of 2642 bp
Application
DNA Molecular Weight Marker XIII (50 bp ladder) allows accurate sizing of DNA fragments generated by PCR or restriction digest, followed by separation on agarose gels. DNA Molecular Weight Marker XIII (50 bp ladder) can be used in conjunction with DNA Molecular Weight Marker XIV (100 bp ladder) for precise size determination.
The fragments have 5′-protruding ends and can be labeled with radioactive nucleotides (e.g.,32P-dTTP or 32P-dGTP) by standard filling-in reactions. End-labeling reactions can be performed with a radioactive or nonradioactive dideoxynucleotide (e.g., DIG-11-ddUTP) and Terminal Transferase.
The fragments have 5′-protruding ends and can be labeled with radioactive nucleotides (e.g.,32P-dTTP or 32P-dGTP) by standard filling-in reactions. End-labeling reactions can be performed with a radioactive or nonradioactive dideoxynucleotide (e.g., DIG-11-ddUTP) and Terminal Transferase.
DNA Molecular Weight Marker for agarose gels; simplifies accurate molecular weight determination of double-stranded DNA fragments generated by:
- Restriction digests
- PCR
- RT-PCR
Sequence
The mixture contains 15 double-stranded DNA fragments with the following base pair lengths (1 base pair = 660 daltons): 50, 100, 150, 200, 250, 300, 350, 400, 450, 500, 550, 600, 650, 700, 750, and an additional band of 2642 bp.
Note: Fragment lengths are derived from computer analysis of the DNA sequence. Depending on the size range of the marker, the smallest fragments will only be visible on overloaded gels. The 250- and 500-bp banding pattern is two to three times brighter.
Note: Fragment lengths are derived from computer analysis of the DNA sequence. Depending on the size range of the marker, the smallest fragments will only be visible on overloaded gels. The 250- and 500-bp banding pattern is two to three times brighter.
Unit Definition
50 μg = 1A260 unit
Physical form
Ready-to-use solution, 250μg/ml, in TE buffer (10mM Tris-HCl, 1mM EDTA, pH 8.0).
Preparation Note
Storage conditions (working solution): 2 to 8 °C
Once thawed we recommend further storage at 2 to 8 °C.
Once thawed we recommend further storage at 2 to 8 °C.
Other Notes
For life science research only. Not for use in diagnostic procedures.
Storage Class Code
12 - Non Combustible Liquids
WGK
nwg
Flash Point(F)
does not flash
Flash Point(C)
does not flash
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