11585762001
Roche
DIG Wash and Block Buffer Set
storage temp.:2-8°C
Synonym(s):
wash and block buffer set, dig
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About This Item
UNSPSC Code:
41105326
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Quality Level
manufacturer/tradename
Roche
greener alternative product characteristics
Designing Safer Chemicals
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Greener Alternative Product
greener alternative category
, Aligned
storage temp.
2-8°C
General description
Except for the Blocking solution, the 10x concentrated buffers are diluted to the desired concentrations with sterile double-distilled water (not included).
Washing, blocking and detection buffers (10x concentrated) for the immunological detection of DIG - (digoxigenin-)labeled probes.
Washing, blocking and detection buffers (10x concentrated) for the immunological detection of DIG - (digoxigenin-)labeled probes.
We are committed to bringing you Greener Alternative Products, which adhere to one or more of The 12 Principles of Greener Chemistry. This product is designed as a safer chemical. The DIG System was established as a sensitive and cost-effective alternative to using radioactivity for the labeling and detection of nucleic acids. There are many available publications that prove the versatility of the DIG System, so use of radio-labeling is no longer the only option for labeling of DNA for hybridization.
Application
The DIG Wash and Block Buffer Set is used at various steps of DIG hybridization and DIG detection.
Packaging
1 set containing 4 components.
Quality
The buffers are autoclaved, filtered and tested for the absence of DNases and RNases according to the current Quality Control procedures. The buffers are function tested according to the standard procedure of the DIG Nucleic Acid Detection Kit.
Preparation Note
Working solution: dATP, dGTP, dTTP mixture:
For one labeling reaction pipette:
1 μl dATP, (vial 2)
1 μl dGTP, (vial 4)
1 μl dTTP, (vial 5)
to a reaction vial.
Note: If the same type of labeled deoxyribonucleoside-triphosphate is used repeatedly, we recommend the preparation of a mixture of equal parts of the other three deoxyribonucleoside-triphosphates for convenience.
Storage conditions (working solution):
For one labeling reaction pipette:
1 μl dATP, (vial 2)
1 μl dGTP, (vial 4)
1 μl dTTP, (vial 5)
to a reaction vial.
Note: If the same type of labeled deoxyribonucleoside-triphosphate is used repeatedly, we recommend the preparation of a mixture of equal parts of the other three deoxyribonucleoside-triphosphates for convenience.
Storage conditions (working solution):
- After the first usage, the buffers should be stored further at 2 to 8 °C. We recommend to store the concentrated Blocking solution in aliquots at -15 to -25 °C.
- When Autoclaved: Stock solution can be stored for several days to a week either unopened at RT or at 2 to 8 °C after opening. Alternatively, it can be stored in aliquots at -15 to -25 °C for up to 6 months.
Other Notes
For life science research only. Not for use in diagnostic procedures.
Kit Components Only
Product No.
Description
- Washing Buffer 10x concentrated
- Maleic acid Buffer 10x concentrated
- Blocking Solution 10x concentrated
- Detection Buffer 10x concentrated
Signal Word
Warning
Hazard Statements
Precautionary Statements
Hazard Classifications
Eye Irrit. 2 - Skin Irrit. 2
Storage Class Code
12 - Non Combustible Liquids
WGK
WGK 3
Flash Point(F)
Not applicabledoes not flash
Flash Point(C)
Not applicabledoes not flash
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