03708152001

Streptavidin Mutein Matrix

Streptavidin, suitable for purification of biotinylated proteins and other biomolecules.

• Synonym(s): Biotin-binding Affinity Resin, Reversible Streptavidin Matrix, Streptavidin Beads
• NACRES: NA.21
• UNSPSC Code: 12352200
• Form: suspension
• Mol wt: 60 kDa (approx.)

Properties

• form: suspension
• mol wt: 60 kDa (approx.)
• packaging: pkg of 5 mL
• manufacturer/tradename: Roche
• parameter: 4-25 °C optimum reaction temp.
• technique(s): biotinylated protein purification: suitable
• matrix: (immobilised onto crosslinked agarose beads)
• shipped in: wet ice
• storage temp.: 2-8°C

Description

General description

Contents
Settled resin of 50% suspension Streptavidin Mutein Matrix.

Streptavidin mutein, immobilized.

The binding of biotin to avidin/streptavidin is the strongest known noncovalent interaction of biological molecules [(K_d 0.6 x 10^-15 M (avidin), 4 x 10^-14 M (streptavidin)]. Strong binding provides a great advantage for certain applications of the derived biotin-avidin/streptavidin technology, such as surface immobilization, blotting technologies, immunoassays, cross-linking studies, and histochemistry. However, the use of immobilized avidin/streptavidin for the purification of biotinylated proteins is limited. In this instance, the extreme affinity of the binding partners creates a drawback of enabling elution only under very harsh conditions that usually destroy the functionality of the protein of interest.
Recombinant streptavidin technology was used to screen for a mutant streptavidin with reduced binding affinity toward biotin. By substituting three amino acids, a mutein was obtained with a biotin dissociation constant of 1.3 x 10^-7 M.
The Streptavidin Mutein Matrix provides this mutein immobilized onto cross-linked agarose beads in a highly stable and regenerable form, which allows purification of biotinylated proteins that results in excellent purity and recovery.

Application

• Streptavidin Mutein Matrix: Is highly suitable for purification of biotinylated proteins
• Has been tested for use with in vitro expressed biotinylated proteins, using the RTS AviTag Biotinylation Kits
• Is convenient for purification of chemically biotinylated proteins
• It was also used in single-step affinity chromatography and affinity chromatography.

Features and Benefits

• Mild purification conditions: Low biotin dissociation constant allows elution of biotinylated proteins under gentle conditions.
• Stable: No significant loss of binding capacity upon storage. The low dissociation constant of the streptavidin mutein is obtained by genetic engineering and not by dissociation of avidin- or streptavidin tetramers.
• Economic: High binding capacity and multiple reuse allows economic handling of the matrix.

Preparation Note

Store at 2 to 8 °C. (Do not freeze!)

Other Notes

For life science research only. Not for use in diagnostic procedures.

Safety Information

• Storage Class: 12 - Non Combustible Liquids
• WGK: WGK 1
• Flash Point (°F): does not flash
• Flash Point (°C): does not flash