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ST1568

Sigma-Aldrich

Anti-DEFB4 Mouse mAb (4C4)

liquid, clone 4C4, Calbiochem®

Synonym(s):

Anti-Defensin β4

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About This Item

UNSPSC Code:
12352203
NACRES:
NA.41

biological source

mouse

Quality Level

100

antibody form

purified antibody

antibody product type

primary antibodies

clone

4C4, monoclonal

form

liquid

does not contain

preservative

species reactivity

human

manufacturer/tradename

Calbiochem®

storage condition

OK to freeze
avoid repeated freeze/thaw cycles

isotype

IgG1

shipped in

wet ice

storage temp.

−20°C

target post-translational modification

unmodified

Gene Information

human ... DEFB4A(1673)

General description

Purified mouse monoclonal antibody. Recognizes the DEFB4 protein.
Recognizes the DEFB4 protein.
This Anti-DEFB4 Mouse mAb (4C4) is validated for use in ELISA, Immunoblotting for the detection of DEFB4.

Immunogen

A recombinant polypeptide corresponding to amino acids 24-65 of human DEFB4, expressed as a GST fusion protein

Application


ELISA (see comments)
Immunoblotting (1-5 g/ml)

Warning

Toxicity: Regulatory Review (Z)

Physical form

In PBS, pH 7.2.

Reconstitution

Following initial thaw, aliquot and freeze (-20°C).

Other Notes

For ELISA, this antibody can be used as the capture antibody; recommended concentration is 10 ng/ml, 1 µg/well, 100 µl total volume. Variables associated with assay conditions will dictate the proper working dilution.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

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Storage Class Code

12 - Non Combustible Liquids

WGK

nwg

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Olivia McGinn et al.
Oncogene, 39(12), 2478-2492 (2020-01-29)
Estrogen receptor (ER) positive breast cancers often contain subpopulations of cells that express the intermediate filament protein cytokeratin 5 (CK5). CK5+ cells are enriched in cancer stem cell (CSC) properties, can be induced by progestins, and predict poor prognosis in
Jessica Finlay-Schultz et al.
Breast cancer research : BCR, 22(1), 68-68 (2020-06-25)
Breast cancer is a highly heterogeneous disease characterized by multiple histologic and molecular subtypes. While a myriad of breast cancer cell lines have been developed over the past 60 years, estrogen receptor alpha (ER)+ disease and some mutations associated with this
L M Fettig et al.
Oncogene, 36(44), 6074-6084 (2017-07-12)
Half of estrogen receptor-positive breast cancers contain a subpopulation of cytokeratin 5 (CK5)-expressing cells that are therapy resistant and exhibit increased cancer stem cell (CSC) properties. We and others have demonstrated that progesterone (P4) increases CK5+ breast cancer cells. We

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