ST1507

Anti-AKR1B10 Mouse mAb (1A6)

liquid, clone 1A6, Calbiochem^®

• Synonym(s): Anti-Aldo-Keto Reductase 1B10
• NACRES: NA.41
• UNSPSC Code: 12352203
• Clone: 1A6, monoclonal
• Species reactivity: human
• Application: —
• Citations: 1

Properties

• biological source: mouse
• Quality Segment: 100
• antibody form: purified antibody
• antibody product type: primary antibodies
• clone: 1A6, monoclonal
• form: liquid
• does not contain: preservative
• species reactivity: human
• manufacturer/tradename: Calbiochem^®
• storage condition: OK to freeze, avoid repeated freeze/thaw cycles
• dilution: (ELISA ; Immunoblotting (1-5 µg/mL); Immunocytochemistry (10 µg/mL); Paraffin Sections (3 µg/mL))
• isotype: IgG2a
• shipped in: wet ice
• storage temp.: −20°C
• target post-translational modification: unmodified
• Gene Information: human ... AKR1B10(57016)

Description

General description

Anti-AKR1B10. Mpuse monoclonal, clone 1A6 recognizes the ~37 kDa AKR1B10 protein in HepG2 and HeLa cells and human colon tissue. It is validated for ELISA, WB, ICC, and paraffin sections.

Purified mouse monoclonal antibody. Recognizes the ~37 kDa AKR1B10 protein.

Recognizes the ~37 kDa AKR1B10 protein in HepG2 and HeLa cells and human colon tissue.

Immunogen

A recombinant polypeptide corresponding to amino acids 76-144 of human AKR1B10, expressed as a GST fusion protein

NA

Application

ELISA (see comments)
Immunoblotting (1-5 g/ml)
Immunocytochemistry (10 g/ml)
Paraffin Sections (3 g/ml)

Physical form

In PBS, pH 7.2.

Preparation Note

Following initial thaw, aliquot and freeze (-20°C).

Analysis Note

Positive Control
HepG2 cells, Human Colon tissue, HeLa cells

Other Notes

Ahn K.H., et al. 2010 JOURNAL OF HEALTH SCIENCE56, 287.
Satow, R., et al. 2010. Clin. Cancer Res.16, 2518.

For ELISA, this antibody can be used as the capture antibody; recommended concentration is 10 ng/ml, 1 µg/well, 100 µl total volume. Variables associated with assay conditions will dictate the proper working dilution.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Toxicity: Regulatory Review (Z)

Safety Information

• Storage Class: 12 - Non Combustible Liquids
• wgk: nwg