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Anti-Ubiquitin Mouse mAb (FK2)

Name: Anti-Ubiquitin Mouse mAb (FK2)
Brand: MM
Price: 11500 CZK
Availability: InStock

liquid, clone FK2, Calbiochem^®

Synonym(s):

Mouse Anti-Ubiquitin, Ubiquitin Detection Antibody

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Size/SKU	Availability	Price
100 μg	Available to ship TODAYfromAreál Kühne+Nagel spol. s r.o.	CZK 11,500.00

About This Item

NACRES:

NA.41

UNSPSC Code:

12352203

Clone:

FK2, monoclonal

Species reactivity:

Application:

—

Citations:

CZK 11,500.00

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Properties

biological source

mouse

Quality Segment

100

antibody form

unpurified

antibody product type

primary antibodies

clone

FK2, monoclonal

form

liquid

contains

≤0.1% sodium azide as preservative

species reactivity (predicted by homology)

all

manufacturer/tradename

Calbiochem^®

storage condition

OK to freeze, avoid repeated freeze/thaw cycles

dilution

(ELISA
Immunoblotting (1:1000)
Immunoprecipitation (s and comments)
Immunofluorescence )

isotype

IgG1

shipped in

wet ice

storage temp.

−20°C

target post-translational modification

unmodified

Description

General description

Mouse monoclonal antibody generated by immunizing Balb/c mice with the specified immunogen and fusing splenocytes with myeloma cells. Recognizes K²⁹-, K⁴⁸-, and K⁶³-linked polyubiquitinylated and monoubiquitinylated proteins.

Recognizes K²⁹-, K⁴⁸-, and K⁶³-linked polyubiquitinylated and monoubiquitinylated proteins, but not free ubiquitin, in Raji cells.

This Anti-Ubiquitin Mouse mAb (FK2) is validated for use in ELISA, Immunoblotting, Immunoprecipitation, Immunofluorescence for the detection of Ubiquitin.

Immunogen

human

unpurified polyubiquitinylated lysozyme

Application

ELISA (see application references)
Immunoblotting (1:1000)
Immunoprecipitation (see application references and comments)
Immunofluorescence (see application references)

Packaging

Please refer to vial label for lot-specific concentration.

Physical form

In PBS.

Preparation Note

Following initial thaw, aliquot and freeze (-20°C). Store diluted antibody at 4°C for up to 1 month.

Raji cells

Other Notes

Does not recognize free ubiquitin. For immunoprecipitation, once the antibody is immobilized, it demonstrates affinity for free ubiquitin as well as for poly- and monoubiquitinylated proteins. Antibody should be titrated for optimal results in individual systems.

Matsumoto, M., et al. 2005. Proteomics 5, 4545.
Boname, J.N., et al. 2001. Immunity 15, 627.
Campbell, V., et al. 2001. Neuron 32, 1013.
Fujimuro, M., et al. 2003. FEBS Letts. 349, 173.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Toxicity: Standard Handling (A)

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This Item	NA57	OP145	OP29
Sigma-Aldrich ST1200 Anti-Ubiquitin Mouse mAb (FK2)	Sigma-Aldrich NA57 Anti-DNA-PK (Ab-2) Mouse mAb (18-2)	Sigma-Aldrich OP145 Anti-MDM2 (Ab-5) Mouse mAb (4B2C1.11)	Sigma-Aldrich OP29 Anti-p53 (Ab-3) (Mutant) Mouse mAb (PAb240)
clone FK2, monoclonal	clone 18-2, monoclonal	clone 4B2C1.11, monoclonal	clone PAb240, monoclonal
antibody form unpurified	antibody form purified antibody	antibody form purified antibody	antibody form purified antibody
biological source mouse	biological source mouse	biological source mouse	biological source mouse
storage temp. −20°C	storage temp. 2-8°C	storage temp. −20°C	storage temp. 2-8°C
isotype IgG1	isotype IgG1	isotype IgG1	isotype IgG1
Quality Level 100	Quality Level 100	Quality Level 100	Quality Level 100

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Storage Class

12 - Non Combustible Liquids

wgk

WGK 2

flash_point_f

Not applicable

flash_point_c