SCT025

BioTracker 519 Green β-Gal Dye

Live cell imaging dye for β-galactosidase (β-gal) used to detect LacZ reporter gene expression and cellular senescence (SA-β-gal).

• Synonym(s): B-galactosidase probe, Beta galactosidase probe, Live cell β-Gal probe, Live cell imaging probe
• UNSPSC Code: 12352207
• NACRES: NA.47
• eCl@ss: 32161000

Properties

• technique(s): cell based assay: suitable
• detection method: fluorometric
• shipped in: ambient
• storage temp.: -10 to -25°C

Description

General description

β-galactosidase, also called beta-gal or β-gal, is a glycoside hydrolase enzyme that catalyzes the hydrolysis of β-galactosides into monosaccharides through the breaking of a glycosidic bond. β-gal is commonly used in molecular biology as a reporter marker to monitor gene expression using the chromogenic X-Gal. β-gal has also been used to measure cellular senescence (SA-β-gal).

The BioTracker 519 Green β-Gal Dye is a fluorescent probe for the detection of β-galactosidase in living cells. It can be applied to fluorescent imaging and selection of cell and tissue transfected with lacZ along as a measurement of cellular senescence. Other applications include gene analysis by fluorescent imaging, monitoring transfection efficiency, and the study of gene promoter or enhancer elements. The dye can stain LacZ expressing HEK293 cells both before and after 3% paraformaldehyde fixation.

Spectral Properties


Absorbance: 497nm


Emission: 519nm

Application

Live cell fluorescent imaging

Research Category
Cell Imaging

Research Sub Category
Live Cell Dye

Physical form

Lyophilized

Preparation Note

Store BioTracker 519 Green β-Gal Dye at -20°C, desiccate and protect from light


Note: Centrifuge vial briefly to collect contents at bottom of vial before opening

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class: 11 - Combustible Solids
• wgk: WGK 3
• flash_point_f: Not applicable
• flash_point_c: Not applicable