SCC281

MN9D Mouse Dopaminergic Neuronal Cell Line

Mouse

• Synonym(s): MN9D Mouse Dopaminergic Neuronal Cell Line
• UNSPSC Code: 41106514
• NACRES: NA.81
• Biological source: mouse
• Growth mode: N/A
• Manufacturer/tradename: Sigma-Aldrich

Properties

• Product Name: MN9D Mouse Dopaminergic Neuronal Cell Line,
• biological source: mouse
• packaging: pkg of >1X10⁶ vials
• manufacturer/tradename: Sigma-Aldrich
• growth mode: N/A
• technique(s): cell culture | mammalian: suitable
• shipped in: dry ice
• storage temp.: ≤ − 140°C

Description

General description

The hybrid MN9D immortalized dopaminergic neuronal cell line was generated by fusion between rostral mesencephalic neurons from a 14-day-old C57BL/6J mouse embryo and N18TG2 neuroblastoma cells, a sympathetic nervous system cancer of A/Jax background.1 MN9D cells produce dopamine (DA) and express tyrosine hydroxylase (TH) as well as aromatic amino acid decarboxylase (AADC).2 MN9D cells readily aggregate with one another or other embryonic brain cells2 and can be transfected using calcium phosphate precipitation3 or Lipofectamine4.Undifferentiated or differentiated MN9D cells are extensively used to model dopaminergic neurons and to test mechanisms and potential therapeutics relevant to the loss of DA neurons in Parkinson′s disease. Differentiated with butyric acid or glial cell line-derived neurotrophic factor (GDNF) followed by butyric acid, MN9D cells only partially recapitulate the electrophysiological properties of midbrain DA neurons. Optimizing MN9D differentiation further using one or a combination of growth or other factors may yield an improved model system for Parkinson′s disease studies in vitro.5

Application

The mechanisms by which central neurons develop axons and establish functional connections with their target cells as well as the role of such connections in cell survival are important developmental neurobiology questions studied using in vitro cell-reaggregation systems, both for central dopaminergic and cholinergic neurons. Hybrid clonal cells facilitate the study of trophic interactions between mesencephalic dopaminergic neurons and their target cells. References1.Choi HK, Won LA, Kontur PJ, Hammond DN, Fox AP, Wainer BH, Hoffmann PC, Heller A. (1991) Immortalization of embryonic mesencephalic dopaminergic neurons by somatic cell fusion. Brain Res 552(1):67–76.2.Choi HK, Won L, Roback JD, Wainer BH, Heller A. (1992) Specific modulation of dopamine expression in neuronal hybrid cells by primary cells from different brain regions. Proc Natl Acad Sci USA 89(19):8943–8947.3.Perez RG, Waymire JC, Lin E, Liu JJ, Guo F, Zigmond MJ. (2002) A role for alpha-synuclein in the regulation of dopamine biosynthesis. J Neurosci 22(8):3090-30994.Cavanaugh JE, Jaumotte JD, Lakoski JM, Zigmond MJ. (2006) Neuroprotective role of ERK1/2 and ERK5 in a dopaminergic cell line under basal conditions and in response to oxidative stress. J Neurosci Res 84(6):1367-13755.Rick CE, Ebert A , Virag T, Bohn MC, Surmeier DJ. (2006) Differentiated dopaminergic MN9D cells only partially recapitulate the electrophysiological properties of midbrain dopaminergic neurons. Dev Neurosci 28(6):528-537.

• Each vial contains ≥ 1X10⁶ viable cells.• MN9D cells are verified to be of mouse origin and negative for rat, Chinese hamster, Golden Syrian hamster, human, and non-human primate interspecies contamination, as assessed by a Contamination Clear panel by Charles River Animal Diagnostic Services.• Cells tested negative for infectious diseases by a Mouse Essential CLEAR panel by Charles River Animal Diagnostic Services.• Cells tested negative for mycoplasma.

Features and Benefits

Undifferentiated or differentiated MN9D cells are extensively used to model dopaminergic neurons and to test mechanisms and potential therapeutics relevant to the loss of DA neurons in Parkinson′s disease.

Preparation Note

Store in liquid nitrogen. The cells can be cultured for at least 10 passages after initial thawing without significantly affecting the cell marker expression and functionality.

Other Notes

Subject to local law, this product is intended to be sold for internal in vitro research use only subject to terms and conditions found here: www.sigmaaldrich.com/restrictedcelluse. This product may not be: re-engineered or copied; used to make derivatives, modifications or functional equivalents; used to obtain patents or other IP claiming use of the product; used to develop, test, or manufacturer a commercial product; used as a component in a commercial product; resold or licensed; used in any clinical applications or trials; or used in humans. A license or limited commercial use agreement is required for use by any for-profit entity, use in services, and use in sponsored academic research. For information regarding any such use, please contact licensing@milliporesigma.com.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class: 12 - Non Combustible Liquids
• wgk: WGK 2
• flash_point_f: Not applicable
• flash_point_c: Not applicable